Mapping of a neutralizing epitope in the surface envelope protein of porcine endogenous retrovirus subgroup B
Mapping of a neutralizing epitope in the surface envelope protein of porcine endogenous retrovirus subgroup B
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猪内源性逆转录病毒B亚型表面包膜蛋白中和表位的定位
DOI：
10.1099/vir.0.029322-0
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发表时间：
2011
期刊：
影响因子：
3.8
通讯作者：
Miyazawa Takayuki
中科院分区：
文献类型：
--
作者：
Nakaya Yuki;Hoshino Shigeki;Yasuda Jiro;Miyazawa Takayuki
猪被认为是最适合异种移植的供体动物。然而，猪的基因组中含有潜在危险的感染因子，称为猪内源性逆转录病毒（PERV）。在本研究中，我们生成了针对 PERV-B 表面 (SU) 包膜蛋白 (Env) 的 mAb，命名为 KRT1。通过对感染 PERV-B 的细胞进行间接免疫荧光测定和流式细胞术分析来检测 KRT1 结合。通过免疫印迹分析，KRT1 中和 PERV-B 假型病毒并特异性识别 PERV-B SU Env，但不识别 PERV-A SU Env。肽-ELISA 显示 KRT1 识别位于富含脯氨酸区域的线性肽序列 (ALEPPHNLPVP)，该区域是 SU Env 的子域之一。总之，KRT1抗体将成为PERV-B研究的有用工具，更重要的是，它可能为异种移植中针对PERV-B感染提供新的保护策略。
Pigs are thought to be the most suitable donor animal for xenotransplantation. However, pigs harbour potentially hazardous infectious agents, termed porcine endogenous retroviruses (PERVs), in their genome. In this study, we generated a mAb against PERV-B surface (SU) envelope protein (Env), designated KRT1. KRT1 binding was detected by an indirect immunofluorescence assay and flow cytometric analysis on cells infected with PERV-B. KRT1 neutralized PERV-B pseudotype virus and specifically recognized PERV-B SU Env, but not PERV-A SU Env by immunoblotting analysis. The peptide-ELISA revealed that KRT1 recognized a linear peptide sequence (ALEPPHNLPVP) residing in a proline-rich region that is one of the subdomains of SU Env. In conclusion, the KRT1 antibody will serve as a useful tool for the study of PERV-B and, more importantly, it may provide new protective strategies against PERV-B infection in xenotransplantation.