Mesoporous silica nanoparticles functionalized with folic acid/methionine for active targeted delivery of docetaxel.
Mesoporous silica nanoparticles functionalized with folic acid/methionine for active targeted delivery of docetaxel.
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DOI：
10.2147/ott.s113815
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发表时间：
2016
影响因子：
4
通讯作者：
Amanlou M
中科院分区：
文献类型：
--
作者：
Khosravian P;Shafiee Ardestani M;Khoobi M;Ostad SN;Dorkoosh FA;Akbari Javar H;Amanlou M
介孔二氧化硅纳米颗粒（MSNs）是一种具有高负载能力和大功能化表面积的靶向递送载体。在本研究中，制备了一系列多西他赛负载叶酸或蛋氨酸功能化的介孔二氧化硅纳米颗粒（DTX/MSN-FA或DTX/MSN-Met），这些纳米颗粒具有大孔隙和内孔表面胺基性质。结果表明，所合成的微孔微球具有良好的有效载荷和ph敏感的药物释放动力学。对MCF-7细胞的细胞毒性和细胞凋亡的研究表明，靶向纳米颗粒的细胞摄取增加。健康BALB/c小鼠的体内荧光成像证实，裸MSN-NH2主要积聚在肝脏，而MSN-FA或MSN-Met更集中在肾脏。重要的是，肿瘤诱导的BALB/c小鼠器官的离体荧光图像显示了MSN-FA到达肿瘤组织的能力。综上所述，DTX/ msn具有良好的抗癌活性，增强了乳腺癌靶向给药的可能性。
Mesoporous silica nanoparticles (MSNs) are known as carriers with high loading capacity and large functionalizable surface area for target-directed delivery. In this study, a series of docetaxel-loaded folic acid- or methionine-functionalized mesoporous silica nanoparticles (DTX/MSN-FA or DTX/MSN-Met) with large pores and amine groups at inner pore surface properties were prepared. The results showed that the MSNs were successfully synthesized, having good pay load and pH-sensitive drug release kinetics. The cellular investigation on MCF-7 cells showed better performance of cytotoxicity and cell apoptosis and an increase in cellular uptake of targeted nanoparticles. In vivo fluorescent imaging on healthy BALB/c mice proved that bare MSN-NH2 are mostly accumulated in the liver but MSN-FA or MSN-Met are more concentrated in the kidney. Importantly, ex vivo fluorescent images of tumor-induced BALB/c mice organs revealed the ability of MSN-FA to reach the tumor tissues. In conclusion, DTX/MSNs exhibited a good anticancer activity and enhanced the possibility of targeted drug delivery for breast cancer.