Proton NMR investigation of the nucleosome core particle: evidence for regions of altered hydrogen bonding.
Proton NMR investigation of the nucleosome core particle: evidence for regions of altered hydrogen bonding.
复制标题
核小体核心颗粒的质子核磁共振研究：氢键改变区域的证据。
DOI：
10.1021/bi00346a002
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发表时间：
1985
期刊：
影响因子：
2.9
通讯作者：
Wilson,WD
中科院分区：
文献类型：
--
作者：
McMurray,CT;vanHolde,KE;Jones,RL;Wilson,WD
Novel'H nuclear magnetic resonance(NMR) resonances, arising from exchangeable protons and centered at~ 11.2 and 10.1 parts per million (ppm), have been observed in the low-field spectrum (10-15 ppm) of the chicken erythrocyte core particle [145±2 base pairs (bp)]. These peaks are located upfield from the normal adenine-thymine (AT) and guanine-cytosine (GC) imino peaks characteristic of B-form deoxyribonucleic acid (DNA) and are not observed in free DNA under identical conditions. The appearance of the new peaks is ionic strength dependent and temperature-reversible below 75 C. At 25 C, the upfield peak area represents 5% of the DNA base pairs (7 bp), while between 45 and 55 C, the area increases to 18%, affecting approximately 25 bp. Area increases in the upfield resonances result in a complementary decrease in the AT and GC imino peaks found between 12 and 14 ppm. We believe these novel proton signals represent a histone-induced DNA conformational change which involves localized alteration of base pairing in the core particle.
