Detection of genogroup IV norovirus in wastewater and river water in Japan
Detection of genogroup IV norovirus in wastewater and river water in Japan
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DOI：
10.1111/j.1472-765x.2009.02718.x
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发表时间：
2009-11-01
影响因子：
2.4
通讯作者：
Ohgaki, S.
中科院分区：
文献类型：
--
作者：
Kitajima, M.;Haramoto, E.;Ohgaki, S.
目的：为了测试在日本的废水和河水基因组IV诺如病毒（GIV NoV）。方法和结果：从污水处理厂和玉川河水样品的流入物和流出物样品，每月收集一年。采用吸附-洗脱法浓缩水样，HA负电性滤膜加酸冲洗，TaqMan实时荧光定量RT-PCR检测GIV NoV。在冬季和春季，废水和河水样品显示出较高的GIV NoV阳性率。废水和河水中的最高浓度分别为6个中心点9 × 104和1个中心点5 × 104拷贝l-1.Conclusions：环境中GIV NoV的存在表明，不仅GI和GII NoV，而且GIV菌株正在循环，建议对水环境中的GIV NoV进行常规监测，以了解其流行病学，环境分布和潜在的健康风险。
Aims:To test wastewater and river water in Japan for genogroup IV norovirus (GIV NoV).Methods and Results:Influent and effluent samples from a wastewater treatment plant and the Tamagawa River water samples were collected monthly for a year. The water samples were concentrated by the adsorption-elution method, using an HA electronegative filter with acid rinse procedure, followed by quantitative detection of GIV NoV using TaqMan-based real-time RT-PCR. Both wastewater and river water samples showed a high positive ratio of GIV NoV during winter and spring. The highest concentration in wastewater and river water was 6 center dot 9 x 104 and 1 center dot 5 x 104 copies l-1, respectively.Conclusions:Presence of GIV NoV in the environments demonstrates that not only GI and GII NoVs but also GIV strains are circulating and that routine monitoring of GIV NoV in water environments is recommended to understand its epidemics, environmental distribution and potential health risks.Significance and Impact of the Study:This is the first study providing quantitative data on the occurrence of GIV NoV in environmental water over a 1-year period.