Evidence that the human cytomegalovirus 46-kDa UL72 protein is not an active dUTPase but a late protein dispensable for replication in fibroblasts
Evidence that the human cytomegalovirus 46-kDa UL72 protein is not an active dUTPase but a late protein dispensable for replication in fibroblasts
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DOI：
10.1016/j.virol.2004.05.010
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发表时间：
2004-08-01
期刊：
影响因子：
3.7
通讯作者：
Gribaudo, G
中科院分区：
文献类型：
--
作者：
Caposio, P;Riera, L;Gribaudo, G
人巨细胞病毒（HCMV）UL 72基因被认为是α-和γ-疱疹病毒的dUTR基因的等价物。为了表征其功能，在RNA和蛋白质水平上测定了UL 72的表达谱。该基因以晚期动力学表达，并且相应的UL 72 46-kDa蛋白在感染期间晚期在感染细胞的细胞质中积累。pUL 72在E.大肠杆菌中表达的重组蛋白未显示出可检测的dUTR活性。在UL 72 ORF内携带缺失的重构HCMV RVDeltaUL 72病毒的病毒产量在低MOI感染后表现出中度生长缺陷，而它们的DNA合成谱与亲本HCMV RVAD 169的DNA合成谱没有显著差异。这些结果表明，UL 72基因产物不是dUTR，并且对于人成纤维细胞中的复制不是必需的。(C)2004年爱思唯尔公司All rights reserved.
The Human Cytomegalovirus (HCMV) UL72 gene is considered to be the equivalent of the dUTPase gene of the Alpha- and Gamma-herpesviruses. To characterize its function, the expression profiles of UL72 at both the RNA and the protein level were determined. The gene is expressed with a late kinetics and the corresponding UL72 46-kDa protein accumulates late during infection in the cytoplasm of infected cells. The pUL72 was expressed in E. coli and the purified recombinant protein did not display a detectable dUTPase activity. The viral yields of reconstituted HCMV RVDeltaUL72 viruses carrying a deletion within the UL72 ORF demonstrated a moderate growth defect following low MOI infections, whereas their DNA synthesis profiles were not significantly different from those of the parental HCMV RVAD169. These results demonstrate that the UL72 gene product is not a dUTPase and is not essential for replication in human fibroblasts. (C) 2004 Elsevier Inc. All rights reserved.