The end-to-side peripheral nerve repair -: Functional and morphometric study using the peroneal nerve of rats
The end-to-side peripheral nerve repair -: Functional and morphometric study using the peroneal nerve of rats
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DOI：
10.1016/j.jneumeth.2003.12.018
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发表时间：
2004-06-15
影响因子：
3
通讯作者：
Barreira, AA
中科院分区：
文献类型：
--
作者：
De Sá, JMR;Mazzer, N;Barreira, AA
在大鼠腓神经损伤模型中，与传统端对端修复相比，研究了端对端修复后的形态和功能恢复。将28只大鼠随机分为4组：（1）神经残端埋入邻近肌肉组（n = 8）;（2）常规端端修复组（n = 7）;（3）胫神经端侧修复组（n = 8）;（4）假手术组（n = 5）。坐骨神经功能指数（SFI）每周进行评估，共8周，在第56天切除腓神经进行组织学和形态计量学研究。第2组（-16.9）和第3组（-22.7）的SFI逐渐改善，但未达到正常值（约-8）。第2组和第3组的平均神经纤维密度均增加至正常值，但伴随着平均最小和最大神经纤维直径、髓鞘面积和G商的显著降低。第2组和第3组或第2组和第4组之间的差异不显著。我们的结论是，虽然导致显着的形态和功能的恢复，端侧修复是不是有效的传统的端端神经修复。然而，端侧修复在人类的特定病例中具有应用潜力。(C)2004 Elsevier B. V.保留所有权利。
Morphologic and functional recovery following an end-to-side repair was studied comparatively with conventional end-to-end repair in a model of peroneal nerve lesion in rats. Twenty-eight rats were used and divided into four groups according to the reparative procedure following nerve division: (1) nerve stumps buried into neighboring muscles (n = 8); (2) conventional end-to-end repair (n = 7); (3) end-to-side repair onto the tibial nerve (n = 8); (4) sham operation (n = 5). The sciatic functional index (SFI) was evaluated at weekly intervals for 8 weeks, the peroneal nerve being resected on the 56th day for histologic and morphometric studies. The SFI progressively improved in Groups 2 (-16.9) and 3 (-22.7), although it did not reach normal values (around -8). The average nerve fiber density increased to normal values in both Groups 2 and 3, although accompanied by a marked decrease of average minimal and maximal nerve fiber diameter, myelin sheath area and G quotient. The differences between Groups 2 and 3 or Groups 2 and 4 were not significant. We conclude that, although resulting in significant morphologic and functional recovery, end-to-side repair is not as efficient as the conventional end-to-end nerve repair. However, end-to-side repair has a potential for application in selected cases in humans. (C) 2004 Elsevier B.V. All rights reserved.