In vitro cultivation of Schistosoma japonicum-parasites and cells.
In vitro cultivation of Schistosoma japonicum-parasites and cells.
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DOI：
10.1016/j.biotechadv.2013.09.003
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发表时间：
2013-12
影响因子：
16
通讯作者：
Qing Ye;Huifen Dong;C. Grevelding;Min Hu
中科院分区：
文献类型：
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作者：
Qing Ye;Huifen Dong;C. Grevelding;Min Hu
血吸虫病是由血吸虫属吸虫引起的一种严重的寄生虫性人畜共患病。了解该寄生虫的基因和蛋白质功能对揭示该病原体的复杂生物学特性具有重要意义，这将为设计新的血吸虫病防治策略提供有价值的信息。据报道，研究血吸虫基因功能的有效分子工具，如抑制剂研究和转基因，依赖于这种寄生虫和细胞的体外培养系统的发展。除了对曼氏血吸虫的体外培养研究外，中国研究人员对日本血吸虫的体外培养也进行了大量优秀的研究，并发表在中国期刊上。s生命周期的几乎每个阶段。采用体外培养方法培养日本血吸虫，包括miracidia、母孢子囊、尾蚴、血吸虫和产卵成虫，同时引入了几种有助于改善培养条件的培养基和补充物。通过体外培养，不仅可以产生母孢子囊，而且可以从尾蚴中获得成虫。完成s的生命周期的主要障碍。在实验室中，日本血吸虫都是从母孢子囊过渡到尾蚴，并产生受精卵，由体外产生的成熟的成虫卵。关于来自ms的细胞。除建立分离方案和形态观察外，还通过不同的化学试剂、生物添加剂和物理处理对培养基进行了优化。其中，诱变剂如n -甲基-n -硝基-n -亚硝基胍和添加细胞外基质可诱导有丝分裂活性。虽然体外培养细胞的酶活性或银染核仁区相关蛋白水平仍不理想，但这一成果表明有可能达到建立s细胞系的目的。日本血吸虫。结果表明，两种菌种培养方法均取得了较好的效果。日本血吸虫及其细胞的获取为今后对这一重要寄生虫的研究提供了良好的进展。
Schistosomiasis is a serious parasitic zoonosis caused by blood-dwelling flukes of the genus Schistosoma. Understanding functions of genes and proteins of this parasite is important for uncovering this pathogen's complex biology, which will provide valuable information to design new strategies for schistosomiasis control. Effective applications of molecular tools reported to investigate schistosome gene function, such as inhibitor studies and transgenesis, rely on the developments ofin vitrocultivation system of this parasite and cells. Besides thein vitroculture studies dealing with Schistosoma mansoni, there are also numerous excellent studies about thein vitrocultivation of Schistosoma japonicum, which were performed by Chinese researchers and published in Chinese journals. Nearly every stage of the life-cycle ofS. japonicum, including miracidia, mother sporocysts, cercariae, schistosomula, and egg-laying adult worms, was employed for developingin vitrocultivation methods, being accompanied by the introduction of several media and supplements that helped to improve culture conditions. It was not only possible to generate mother sporocysts from miracidiain vitro, but also to obtain adult worms from cercariae throughin vitrocultivation. The main obstacles to complete the life cycle ofS. japonicumin the lab are the transition from mother sporocysts to cercariae, and the production of fertilized and completely developed eggs by adult worms generatedin vitro. With regard to cells fromS. japonicum, besides established isolation protocols and morphological observations, media optimizations were conducted by using different chemical reagents, biological supplements and physical treatment. Among these, mutagens like N-methyl-N-nitro-N-nitrosoguanidine and the addition of extracellular matrix were found to be able to induce mitogenic activities. Although enzyme activities or the level of silver-stained nucleolar region associated protein in cultured cells indicated still suboptimal conditions, the achievements made point to the possibility of reaching the aim of establishing cell lines forS. japonicum. Both the improvements of thein vitroculture of larval and adult worms ofS. japonicumas well as the access of cells of this parasite provide excellent advances for research on this important parasite in the future.