Simplified technique for heterotopic vascularized cervical heart transplantation in mice
Simplified technique for heterotopic vascularized cervical heart transplantation in mice
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DOI：
10.1002/micr.20082
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发表时间：
2005-01-01
期刊：
影响因子：
2.1
通讯作者：
Li, XK
中科院分区：
文献类型：
--
作者：
Wang, QX;Liu, YS;Li, XK
技术问题限制了小鼠血管化心脏移植作为医学研究工具的广泛应用。在这份报告中，我们描述了一个简单的方法进行异位颈部移植的袖口技术。供体心脏的右肺动脉配有22号袖带。在头臂动脉水平分离并横切主动脉弓。在用9-0丝线近端结扎下腔静脉后，近端结扎残余血管和肺，然后小心横切。然后将颈动脉外翻到Teflon套囊上。从供体心脏的获取到受体小鼠皮肤缝合的整个过程可在35 min内完成。这种简化的小鼠心脏移植方法成功率高，可用于移植免疫学研究。(C)2004 Wiley-Liss，Inc.
Technical problems have limited the widespread use of mouse vascularized heart transplantation as a medical research tool. In this report, we describe a simplified method for performing heterotopic cervical transplantation by the cuff technique. The right pulmonary artery of the donor heart is equipped with a 22-gauge cuff. The aortic arch is isolated and transected at the level of the brachiocephalic artery. After proximally ligating the inferior vena cava with 9-0 silk, the residual blood vessels and lungs are ligated proximally and then carefully transected. The carotid artery is then everted over the Teflon cuff. Using this modified procedure, the operation from harvesting the donor heart to skin closure of the recipient mouse can be completed within 35 min. This simplified method for mouse heart transplantation was shown to have a high success rate, and is practical for use in transplantation immunology research. (C) 2004 Wiley-Liss, Inc.