Role of the RecBCD recombination pathway in Salmonella virulence
Role of the RecBCD recombination pathway in Salmonella virulence
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DOI：
10.1128/jb.184.2.592-595.2002
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发表时间：
2002-01-01
影响因子：
3.2
通讯作者：
Casadesús, J
中科院分区：
文献类型：
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作者：
Cano, DA;Pucciarelli, MG;Casadesús, J
缺乏RecBC功能的肠沙门氏菌突变体在小鼠中是无毒的，并且不能在巨噬细胞内生长（N.A. Buchmeier， C.J. Lipps, m.y.h So， F. Heffron, Mol. microsofl .,1993）。RecBC（-）突变体的毒力相关缺陷不受sbcB和sbcCD突变的抑制，表明RecF重组途径的激活不能取代RecBCD的毒力相关功能。RecF通路的功能，如RecJ和RecF，不是毒性所必需的。由于已知RecBCD途径而非RecF途径参与DNA复制过程中产生的双链断裂的修复，因此我们提出肠球菌的全身性感染可能需要RecBCD介导的重组修复来启动吞噬细胞内的DNA复制。缺乏ReeD和RecJ的突变体在小鼠中也被减弱，并且不能在巨噬细胞中增殖。表明外切酶V和IX在沙门氏菌感染期间为recbcd介导的重组修复提供了替代功能。
Mutants of Salmonella enterica lacking the RecBC function are avirulent in mice and unable to grow inside macrophages (N.A. Buchmeier, C.J. Lipps, M. Y. H. So, and F. Heffron, Mol. Microbiol. 7:933-936,1993). The virulence-related defects of RecBC(-) mutants are not suppressed by sbcB and sbcCD mutations, indicating that activation of the RecF recombination pathway cannot replace the virulence-related function(s) of RecBCD. Functions of the RecF pathway such as RecJ and RecF are not required for virulence. Since the RecBCD pathway, but not the RecF pathway, is known to participate in the repair of double-strand breaks produced during DNA replication, we propose that systemic infection by S. enterica may require RecBCD-mediated recombinational repair to prime DNA replication inside phagocytes. Mutants lacking both ReeD and RecJ are also attenuated in mice and are unable to proliferate in macrophages.,, suggesting that exonucleases V and IX provide alternative functions for RecBCD-mediated recombinational repair during Salmonella infection.