A Cellular Memory of Developmental History Generates Phenotypic Diversity in C. elegans
A Cellular Memory of Developmental History Generates Phenotypic Diversity in C. elegans
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DOI：
10.1016/j.cub.2009.11.035
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发表时间：
2010-01-26
期刊：
影响因子：
9.2
通讯作者：
Sengupta, Piali
中科院分区：
文献类型：
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作者：
Hall, Sarah E.;Beverly, Matthew;Sengupta, Piali
早期生活经历对成年表型有重大影响[1-3]。然而，动物保留早期经验的细胞记忆的机制还不清楚。在这里，我们表明，成年野生型秀丽隐杆线虫，短暂地通过抗应激的dauer幼虫阶段表现出不同的基因表达谱和生活史特征，相比，绕过这个阶段的成年动物。使用染色质免疫沉淀实验结合大规模平行测序，我们发现，特定组蛋白尾部修饰的全基因组水平显着改变postdauer动物。在postdauer动物中，与染色质重塑有关的基因亚群的突变消除或改变了所观察到的基因表达和生活史特征的变化。由于早期经历而导致的表观基因组的修饰可能部分地有助于对早期经历的记忆，并在同基因群体中产生表型变异。
Early life experiences have a major impact on adult phenotypes [1-3]. However, the mechanisms by which animals retain a cellular memory of early experience are not well understood. Here we show that adult wild-type Caenorhabditis elegans that transiently pass through the stress-resistant dauer larval stage exhibit distinct gene expression profiles and life history traits, as compared to adult animals that bypassed this stage. Using chromatin immunoprecipitation experiments coupled with massively parallel sequencing, we found that genome-wide levels of specific histone tail modifications are markedly altered in postdauer animals. Mutations in subsets of genes implicated in chromatin remodeling abolish, or alter, the observed changes in gene expression and life history traits in postdauer animals. Modifications to the epigenome as a consequence of early experience may contribute in part to a memory of early experience and generate phenotypic variation in an isogenic population.