Sensor histidine kinases mediate ABA and osmostress signaling in the moss Physcomitrium patens
Sensor histidine kinases mediate ABA and osmostress signaling in the moss Physcomitrium patens
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DOI：
10.1016/j.cub.2021.10.068
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发表时间：
2021-11
期刊：
影响因子：
9.2
通讯作者：
Tsukasa Toriyama;Akihisa Shinozawa;Yuki Yasumura;Masashi Saruhashi;Mayuka Hiraide;Shiori Ito;H. Matsuura;K. Kuwata;M. Yoshida;T. Baba;Izumi Yotsui;T. Taji;D. Takezawa;Y. Sakata
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文献类型：
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作者：
Tsukasa Toriyama;Akihisa Shinozawa;Yuki Yasumura;Masashi Saruhashi;Mayuka Hiraide;Shiori Ito;H. Matsuura;K. Kuwata;M. Yoshida;T. Baba;Izumi Yotsui;T. Taji;D. Takezawa;Y. Sakata
为了在陆地上不断变化的水资源供应中生存下来，陆地植物必须能够感知干旱和洪水等水分压力。植物激素脱落酸(ABA)和植物特有的SNF1相关蛋白激酶2(SnRK2)在植物的渗透胁迫反应中起着关键作用。我们最近报道，在苔藓中，ABA和依赖渗透胁迫的SnRK2的激活需要上游的RAF样激酶(ARK)的磷酸化。这个RAF/SnRK2模块是陆地植物渗透胁迫信号的进化保守机制。令人惊讶的是，ARK也是拟南芥CONSTITUTIVE Response 1(CTR1)的同源基因，CTR1负调控乙烯介导的淹没反应OFP。专利，表明调节对水分供应的反应的两个信号通路之间存在相互作用。然而，ARK/SnRK2模块被激活以响应水分胁迫的机制仍有待阐明。在这里，我们证明了一组乙烯受体相关的组氨酸激酶(ETR-HKS)在ABA和渗透压反应中是必不可少的。Ph来源的ETR-HK的胞内激酶结构域在内质网平台上与Ark相互作用。此外，HK干扰物缺乏ABA依赖的ARK激活环中关键丝氨酸残基的自动磷酸化，导致SnRK2在ABA和渗透胁迫下失去激活。总体来说，ETR-HKS参与了淹没响应，我们目前的数据表明，HK/Ark模块作为环境水可获得性的整合单位，在苔藓植物中诱导优化的水分胁迫响应。
To survive fluctuating water availability on land, terrestrial plants must be able to sense water stresses, such as drought and flooding. The plant hormone abscisic acid (ABA) and plant-specific SNF1-related protein kinase 2 (SnRK2) play key roles in plant osmostress responses. We recently reported that, in the mossPhyscomitrium patens, ABA and osmostress-dependent SnRK2 activation requires phosphorylation by an upstream RAF-like kinase (ARK). This RAF/SnRK2 module is an evolutionarily conserved mechanism of osmostress signaling in land plants. Surprisingly, ARK is also an ortholog ofArabidopsisCONSTITUTIVE RESPONSE 1 (CTR1), which negatively regulates the ethylene-mediated submergence response ofP. patens, indicating a nexus for cross-talk between the two signaling pathways that regulate responses to water availability. However, the mechanism through which the ARK/SnRK2 module is activated in response to water stress remains to be elucidated. Here, we show that a group of ethylene-receptor-related sensor histidine kinases (ETR-HKs) is essential for ABA and osmostress responses inP. patens. The intracellular kinase domain of an ETR-HK fromP. patensphysically interacts with ARK at the endoplasmic reticulumin planta. Moreover,HKdisruptants lack ABA-dependent autophosphorylation of the critical serine residue in the activation loop of ARK, leading to loss of SnRK2 activation in response to ABA and osmostress. Collectively with the notion that ETR-HKs participate in submergence responses, our present data suggest that the HK/ARK module functions as an integration unit for environmental water availability to elicit optimized water stress responses in the mossP. patens.