Notch4 is activated in endothelial and smooth muscle cells in human brain arteriovenous malformations.
Notch4 is activated in endothelial and smooth muscle cells in human brain arteriovenous malformations.
复制标题
DOI：
10.1111/jcmm.12115
复制
发表时间：
2013-11
影响因子：
5.3
通讯作者：
Jin K
中科院分区：
文献类型：
--
作者：
ZhuGe Q;Wu Z;Huang L;Zhao B;Zhong M;Zheng W;GouRong C;Mao X;Xie L;Wang X;Jin K
Notch 4在小鼠动静脉畸形（AVM）的内皮细胞中表达上调。然而，Notch4是否也参与人类脑AVM仍不清楚。在这里，我们对正常脑血管组织和手术切除的脑AVM进行免疫组织化学，发现Notch4在脑AVM病灶的异常血管亚组中上调，与对照脑血管组织相比。双光子共聚焦图像显示Notch 4不仅在脑AVM血管壁的内皮细胞中表达，而且在血管壁的平滑肌细胞中也表达。免疫组化证实Notch4在正常人脑大脑中动脉无表达，而在脑动静脉畸形中Notch4有表达。我们的研究结果表明，Notch4信号转导可能有助于人类脑AVM的发展。
Up-regulation of Notch4 was observed in the endothelial cells in the arteriovenous malformations (AVMs) in mice. However, whether Notch4 is also involved in brain AVMs in humans remains unclear. Here, we performed immunohistochemistry on normal brain vascular tissue and surgically resected brain AVMs and found that Notch4 was up-regulated in the subset of abnormal vessels of the brain AVM nidus, compared with control brain vascular tissue. Two-photon confocal images show that Notch4 was expressed not only in the endothelial but also in the smooth muscle cells of the vascular wall in brain AVMs. Western blotting shows that Notch4 was activated in brain AVMs, but not in middle cerebral artery of normal human brain, which was confirmed by immunostaining. Our findings suggest a possible contribution of Notch4 signalling to the development of brain AVMs in human.
登录
查看更多内容
DOI：
10.1038/labinvest.2009.62
发表时间：
2009-09
期刊：
Laboratory investigation; a journal of technical methods and pathology
影响因子：
--
作者：
通讯作者：
--
影响因子：
4.1
作者：
Mullan, S;Mojtahedi, S;Macdonald, RL
通讯作者：
Macdonald, RL
影响因子：
4.8
作者：
Uranishi, R;Baev, NI;Awad, IA
通讯作者：
Awad, IA
DOI：
10.1073/pnas.0611177104
发表时间：
2007-02-27
影响因子：
11.1
作者：
Suchting, Steven;Freitas, Catarina;Eichmann, Anne
通讯作者：
Eichmann, Anne
影响因子：
8.3
作者：
Kim, Grace H.;Hahn, David K.;Connolly, Sander, Jr.
通讯作者：
Connolly, Sander, Jr.