Circular dichroism probed by two-photon fluorescence microscopy in enantiopure chiral polyfluorene thin films.
Circular dichroism probed by two-photon fluorescence microscopy in enantiopure chiral polyfluorene thin films.
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DOI：
10.1021/ja209916y
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发表时间：
2012-03
影响因子：
15
通讯作者：
M. Savoini;Xiaofei Wu;M. Celebrano;J. Ziegler;P. Biagioni;S. Meskers;L. Duò;B. Hecht;M. Finazzi
中科院分区：
文献类型：
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作者：
M. Savoini;Xiaofei Wu;M. Celebrano;J. Ziegler;P. Biagioni;S. Meskers;L. Duò;B. Hecht;M. Finazzi
双光子荧光扫描共聚焦显微镜敏感的圆二色性与衍射极限分辨率远低于500 nm的证明。用这种方法，热退火的手性聚芴薄膜的圆二色性的空间变化已被成像。我们观察到圆二色性与亚微米大小的域显示螺旋扭曲的大分子组织。与左手或右手分子组织相对应的具有相反手性性质的域共存于膜中。我们的结果是一致的，通过单光子成像，并说明了潜在的双光子成像用于研究螺旋大分子组织。
Two-photon fluorescence scanning confocal microscopy sensitive to circular dichroism with a diffraction-limited resolution well below 500 nm is demonstrated. With this method, the spatial variation of the circular dichroism of thermally annealed chiral polyfluorene thin films has been imaged. We observed circular dichroism associated with submicrometer-sized domains showing helicoidally twisted macromolecular organization. Domains with opposite chiroptical properties, corresponding to left- or right-handed molecular organization, coexist in the film. Our results are consistent with those obtained by one-photon imaging and illustrate the potential of two-photon imaging for use in studying helical macromolecular organization.