Levels of mRNAs which code for small, acid-soluble spore proteins and their LacZ gene fusions in sporulating cells of Bacillus subtilis.
Levels of mRNAs which code for small, acid-soluble spore proteins and their LacZ gene fusions in sporulating cells of Bacillus subtilis.
复制标题
枯草芽孢杆菌孢子细胞中编码小型酸溶性孢子蛋白及其 LacZ 基因融合体的 mRNA 水平。
DOI：
10.1093/nar/16.14.6567
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发表时间：
1988
影响因子：
14.9
通讯作者：
Setlow,P
中科院分区：
文献类型：
--
作者：
Mason,JM;Fajardo-Cavazos,P;Setlow,P
来自基因（sspA，BandE.）编码枯草芽孢杆菌主要的小的酸溶性孢子蛋白的基因，以及sp-lacZ基因融合体的mRNA水平。增加ssp-lacZ融合基因的基因剂量导致了sp-lacZ mRNA水平和b-半乳糖苷酶积累速率的平行增加。类似地，spE基因剂量增加11倍，spEmRNA也相应增加，但积累的spE基因产物量最多增加1.5倍。相比之下，spAorB基因剂量增加11倍对总spAplussspBmRNA水平没有显著影响，但确实改变了这些mRNA的比例及其基因产物的量，以反映两种基因比例的改变。这些结果表明，完整的sp基因，而不是ssp-lacZ基因融合，受到反馈调节的基因表达，与这种调节的thesspA和B基因的影响，通过mRNA水平的调制，而反馈调节的thesspE基因是在转录后水平。
The levels of mRNAs from genes (sspA,BandE.) which code for major small, acid-soluble, spore proteins ofBacillussubtilishave been determined, as well as the levels of mRNAs fromssp-lacZgene fusions. Increasing the gene dosage ofssp-lacZfusions resulted in parallel increases in both thessp-lacZmRNA level and the rate of b-galactosidase accumulation. Similarly, an 11-fold increase insspEgene dosage gave a comparable increase insspEmRNA, but at most a 1.5-fold increase in the amount ofsspEgene product accumulated. In contrast, an 11-fold increase in the dosage of thesspAorBgenes had no significant effect on the level of totalsspAplussspBmRNA, but did alter the ratios of these mRNAs as well as the amount of their gene products, to reflect the altered ratio of the two genes. These results suggest that intactsspgenes, but notssp-lacZgene fusions, are subject to feedback regulation of gene expression, with this regulation of thesspAandBgenes effected by modulation of mRNA levels, while the feedback regulation of thesspEgene is at the post-transcriptional level.