Exploitation of the Medfly Gut Microbiota for the Enhancement of Sterile Insect Technique: Use of Enterobacter sp. in Larval Diet-Based Probiotic Applications.
Exploitation of the Medfly Gut Microbiota for the Enhancement of Sterile Insect Technique: Use of Enterobacter sp. in Larval Diet-Based Probiotic Applications.
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DOI：
10.1371/journal.pone.0136459
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Bourtzis K
中科院分区：
文献类型：
--
作者：
Augustinos AA;Kyritsis GA;Papadopoulos NT;Abd-Alla AM;Cáceres C;Bourtzis K
地中海实蝇Ceratitis capitata（Mediterranean fruit fly，Medfly）是一种具有重要经济意义的害虫，也是实蝇科昆虫不育技术（Sterile insect technique，SIT）的模式昆虫。后者部分是由于该物种的遗传性别鉴定品系（GSS）的开发和利用，如维也纳8品系，目前在世界各地的大规模饲养设施中使用。提高这种菌株在大规模饲养设施和田间的性能可以显着提高SIT的功效并降低运营成本。最近的研究表明，操纵肠道共生体可以对昆虫品系的整体适应性产生显着的积极影响。我们使用基于培养的方法来分离和表征在大量饲养条件下的维也纳8菌株的肠道相关细菌种类。我们还利用了一种分离的细菌，肠杆菌属，作为饲料补充剂（益生菌）的幼虫饮食，我们评估了其对健身参数的影响下，在SIT操作程序中使用的标准操作程序。益生菌的应用肠杆菌。导致蛹和成人生产力的改善，以及减少饲养时间，特别是男性，而不影响蛹的重量，性别比，男性交配竞争力，飞行能力和寿命饥饿。
The Mediterranean fruit fly (medfly), Ceratitis capitata, is a pest of worldwide substantial economic importance, as well as a Tephritidae model for sterile insect technique (SIT) applications. The latter is partially due to the development and utilization of genetic sexing strains (GSS) for this species, such as the Vienna 8 strain, which is currently used in mass rearing facilities worldwide. Improving the performance of such a strain both in mass rearing facilities and in the field could significantly enhance the efficacy of SIT and reduce operational costs. Recent studies have suggested that the manipulation of gut symbionts can have a significant positive effect on the overall fitness of insect strains. We used culture-based approaches to isolate and characterize gut-associated bacterial species of the Vienna 8 strain under mass rearing conditions. We also exploited one of the isolated bacterial species, Enterobacter sp., as dietary supplement (probiotic) to the larval diet, and we assessed its effects on fitness parameters under the standard operating procedures used in SIT operational programs. Probiotic application of Enterobacter sp. resulted in improvement of both pupal and adult productivity, as well as reduced rearing duration, particularly for males, without affecting pupal weight, sex ratio, male mating competitiveness, flight ability and longevity under starvation.