Multistep Consolidated Phototherapy Mediated by a NIR-Activated Photosensitizer
Multistep Consolidated Phototherapy Mediated by a NIR-Activated Photosensitizer
复制标题
由近红外激活光敏剂介导的多步综合光疗
DOI：
10.1021/acsami.9b10605
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发表时间：
2019
影响因子：
9.5
通讯作者：
Weian Zhang
中科院分区：
文献类型：
--
作者：
Yudong Xue;Jipeng Li;Guoliang Yang;Zhiyong Liu;Huifang Zhou;Weian Zhang
单一分子在单一治疗功能纳米系统上的多功能作用对提高诊断的准确性和改善治疗效果具有重要意义。因此，我们成功地制备了一种生物相容的多步光疗体系(PPA-Cy7-PEG-Biotin)，其中含有光敏剂焦脱镁叶绿酸A(PPA)和近红外(NIR)菁染料(Cy7)的共价结合物(Cy7)，并将其与生物素功能化，用于灵活的特异性肿瘤靶向光疗。这些胶束在近红外照射后会通过花菁的光降解、光热转化和光控制释放来解聚，以恢复猝灭的PPA的光动力学功能。相继，光敏分子的促进治疗大大延长了肿瘤的滞留时间和治疗效率，在体外和体内都具有多步骤的抗肿瘤作用。与仅作用于肿瘤表面区域的简单光疗配置不同，在相同剂量下，多步骤治疗可以广泛损害肿瘤的浅层和深层区域。因此，与一般的联合光疗方法不同，该策略提出了一种基于光激活的多步光疗平台，在癌症的增强联合光疗中具有巨大的潜力。
The multifunctional effect of a single molecule for therapeutic functionalities on a single theranostic nanosystem has a great significance to enhance the accuracy of diagnosis and improve the efficacy of therapy. Herein, a biocompatible multistep phototherapeutic system (Ppa-Cy7-PEG-biotin) that contains a photosensitizer pyropheophorbide A (Ppa) with the covalent conjunction of a near-infrared (NIR) cyanine dye (Cy7) was successfully fabricated and functionalized with biotin for flexible specific tumor-targeting phototherapy. These theranostic micelles will disaggregate after NIR irradiation via the photodegradation of cyanine accompanied by the photothermal conversion and the optically controlled release for the restoration of photodynamic function of quenched Ppa. Consecutively, promoted treatments of photosensitive molecules greatly prolonged the tumor retention time and treatment efficiency, having a multistep antitumor effect both in vitro and in vivo. Different from the simple phototherapeutic configurations that only act on the superficial areas of tumors at mild doses, the multistep therapy can be competent for broadly damaging the superficial and deeper regions of tumors at the same dose. Therefore, as opposed to the general combination phototherapeutic approach, this strategy presents a photoactivation-based multistep phototheranostic platform with an enormous potential in enhanced combined phototherapy for cancer.