Fine mapping and candidate gene analysis of dense and erect panicle 3, DEP3, which confers high grain yield in rice (Oryza sativa L.)
Fine mapping and candidate gene analysis of dense and erect panicle 3, DEP3, which confers high grain yield in rice (Oryza sativa L.)
复制标题
DOI：
10.1007/s00122-011-1543-6
复制
发表时间：
2011-05-01
影响因子：
5.4
通讯作者：
Koh, Hee-Jong
中科院分区：
文献类型：
--
作者：
Qiao, Yongli;Piao, Rihua;Koh, Hee-Jong
花序构型是水稻的一个重要农艺性状，主要由一次花序分枝和二次花序分枝的形态、数量和长度决定。在本研究中，我们描述了一个新的密集和直立穗（EP）突变体，dep 3，来自水稻ssp。用N-甲基-N-亚硝基脲处理粳稻品种华清。dep 3突变体的穗从开花到完全成熟保持直立，而野生型植物的穗在开花后开始下垂。dep 3突变还调节了穗长、粒形和每穗粒数等穗部性状。解剖学观察表明，dep 3突变体有更多的小维管束和更厚的秆比野生型植物，解释了EP表型。遗传分析表明，密穗型和极早熟型受一对隐性基因控制，命名为dep 3。通过图位克隆的方法，DEP 3基因被鉴定为候选基因，并被预测编码一个patatin样磷脂酶A2（PLA 2）超家族结构域的蛋白。该突变等位基因在LOC_Os06g46350内携带408 bp的基因组缺失，其包括第三外显子的最后47 bp编码区和3 '非翻译区的前361 bp。以上结果表明，类patatin-like PLA 2可能在维管束形成中起重要作用，而dep 3突变体可能为水稻育种提供另一种EP资源。
Architecture of the rice inflorescence, which is determined mainly by the morphology, number and length of primary and secondary inflorescence branches, is an important agronomical trait. In the current study, we characterized a novel dense and erect panicle (EP) mutant, dep3, derived from the Oryza sativa ssp. japonica cultivar Hwacheong treated with N-methyl-N-nitrosourea. The panicle of the dep3 mutant remained erect from flowering to full maturation, whereas the panicle of the wild type plant began to droop after flowering. The dep3 mutation also regulated other panicle characteristics, including panicle length, grain shape and grain number per panicle. Anatomical observations revealed that the dep3 mutant had more small vascular bundles and a thicker culm than wild type plants, explaining the EP phenotype. Genetic analysis indicated that the phenotype with the dense and EP was controlled by a single recessive gene, termed dep3. The DEP3 gene was identified as the candidate via a map-based cloning approach and was predicted to encode a patatin-like phospholipase A2 (PLA2) superfamily domain-containing protein. The mutant allele gene carried a 408 bp genomic deletion within LOC_Os06g46350, which included the last 47 bp coding region of the third exon and the first 361 bp of the 3'-untranslated region. Taken together, our results indicated that the patatin-like PLA2 might play a significant role in the formation of vascular bundles, and that the dep3 mutant may provide another EP resource for rice breeding programs.