A novel chitosan CpG nanoparticle regulates cellular and humoral immunity of mice.
A novel chitosan CpG nanoparticle regulates cellular and humoral immunity of mice.
复制标题
新型壳聚糖 CpG 纳米颗粒可调节小鼠的细胞和体液免疫。
DOI：
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
R. Gao
中科院分区：
文献类型：
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作者：
Kaiyuan Wu;Mei Wu;Manliang Fu;Hui Li;Yi Yang;Huan Zhang;C. Cheng;Zezhou Wang;Xiu;Xuebin Lü;Di Liu;Hua Li;R. Gao
目的
目的研制一种安全、新型的免疫佐剂，提高动物对大肠杆菌的免疫力和抵抗力。大肠杆菌感染。
方法
合成了一个含11个CpG基序的88碱基免疫刺激性寡核苷酸（CpG ODN）。采用离子交联法制备了壳聚糖纳米粒（CNP），并将其包裹于CpG ODN中，在体外对猪淋巴细胞的增殖有显著的促进作用。将CpG-CNP接种于21日龄昆明小鼠，经口攻击K88/K99大肠杆菌强毒。接种后35天。分别于接种后0、7、14、21、28、35、42、49 d从小鼠尾静脉采血，ELISA法检测免疫球蛋白、细胞因子和免疫细胞的变化及含量，如IgG、伊加、IgM、IL-2、IL-4、IL-6等。
结果
与对照组相比，CpG组在体外能显著促进猪淋巴细胞增殖（P < 0.05）。CpG-CNP可显著提高免疫小鼠血清中IgG、IgM和伊加的含量（P < 0.05）。免疫小鼠IL-2、IL-4、IL-6水平显著高于对照组（P < 0.05），白色细胞和淋巴细胞数量也显著高于对照组（P < 0.05）。免疫小鼠的体液免疫和细胞免疫功能明显增强，对大肠杆菌的感染有抵抗作用。对照组小鼠出现明显的感染症状和病变。
结论
CpG-CNP能显著增强小鼠的细胞免疫和体液免疫功能，增强小鼠对大肠杆菌的抵抗力。可作为一种有效的佐剂，提高猪对传染病的免疫保护力和抵抗力。
OBJECTIVE
To develop a safe and novel immunoadjuvant to enhance the immunity and resistance of animals against E. coli infection.
METHODS
An 88-base immunostimulatory oligodeoxynuleotide containing eleven CpG motifs (CpG ODN) was synthesized and amplified by PCR. The chitosan nanoparticle (CNP) was prepared by ion linking method to entrap the CpG ODN that significantly promotes the proliferation of lymphocytes of pig in vitro. Then the CpG-CNP was inoculated into 21-day old Kunming mice, which were orally challenged with virulent K88/K99 E. Coli 35 days after inoculation. Blood was collected from the tail vein of mice on days 0, 7, 14, 21, 28, 35, 42, and 49 after inoculation to detect the changes and content of immunoglobulins, cytokines and immune cells by ELISA, such as IgG, IgA, IgM, IL-2, IL-4, and IL-6.
RESULTS
The CpG provoked remarkable proliferation of lymphocytes of pig in vitro in comparison with that of control group (P < 0.05). The inoculation with CpG-CNP significantly raised the content of IgG, IgM, and IgA in the sera of immunized mice (P < 0.05). The levels of IL-2, IL-4, and IL-6 in the mice significantly increased in comparison with those in controls (P < 0.05), so was the number of white blood cells and lymphocytes in immunized mice. The humoral and cellular immunities were significantly enhanced in immunized mice, which resisted the infection of E. coli and survived, while the control mice manifested evident symptoms and lesions of infection.
CONCLUSIONS
CpG-CNP can significantly promote cellular and humoral immunity and resistance of mice against E. coil infection, and can be utilized as an effective adjuvant to improve the immunoprotection and resistance of porcine against infectious disease.
影响因子：
10.8
作者：
Mao, HQ;Roy, K;Leong, KW
通讯作者：
Leong, KW