Coating and fusing cell membranes onto a silica surface and their chromatographic characteristics
Coating and fusing cell membranes onto a silica surface and their chromatographic characteristics
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DOI：
10.1007/bf02491836
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发表时间：
2001-07-01
期刊：
影响因子：
1.7
通讯作者：
Geng, XD
中科院分区：
文献类型：
--
作者：
He, LC;Wang, SC;Geng, XD
本文报道了一种新型的细胞膜固定相（CMSP），它是在多孔硅胶表面涂敷活性细胞膜，用于亲和色谱。通过将二氧化硅浸入细胞膜的悬浮液中，由于硅醇基团（Si-OH）在二氧化硅表面上的不可逆吸附和细胞膜的自融合，二氧化硅的整个表面被细胞膜覆盖。CMSP可直接用作色谱填料，无需任何额外的化学改性。研究了CMSP的表面性质、酶活性和色谱行为。扫描电子显微镜、表面能谱仪、酶活性测定和液相色谱分析结果表明，CMSP的表面特征与正相和反相固定相有很大的不同。CMSP同时具有细胞膜活性和色谱分离特性。此外，CMSP作为手性固定相，可用于（+/-）Bay-K8644对映体的拆分。某些钙拮抗剂对CMSP的容量因子与其药理作用有较好的相关性。结论：CMSP不仅可作为生物亲和色谱的填料，而且可作为研究药物与受体相互作用的工具。
A new cell membrane stationary phase (CMSP) consisting of porous silica coated with active cell membranes is presented for affinity chromatography. By immersing silica into a suspension of cell membranes, the whole surface of silica was covered by the cell membranes due to the irreversible adsorption of silanol groups (Si-OH) on the silica surface and the self-fusion of the cell membranes. CMSP can be used directly as a chromatographic packing material without any additional chemical modification. The surface characteristics, enzymatic activity, and chromatographic behavior of CMSP were investigated. The results obtained from scanning electron microscope, surface energy spectrometer, enzyme assay, and liquid chromatography showed that the surface characteristics of CMSP were very different from that of normal and reversed stationary phases. CMSP was found to have the characteristics of both cell membrane activity and chromatographic separation. Moreover, CMSP, as a chiral stationary phase, could be used for the enantiomeric separation of (+/-) Bay-K8644. The capacity factor of some calcium antagonists on CMSP was found to have a good correlation with their pharmacological actions. It is concluded that CMSP may be used not only as a kind of packing material in bio-affinity chromatography, but also as a tool for studying the interactions between a drug and its receptor.