Isolation of beta-N-acetylhexosaminidase from rabbit semen and its role in fertilization.
Isolation of beta-N-acetylhexosaminidase from rabbit semen and its role in fertilization.
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从兔精液中分离β-N-乙酰己糖胺酶及其在受精中的作用。
DOI：
10.1042/bj1910827
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
Srivastava,PN
中科院分区：
文献类型：
--
作者：
Farooqui,AA;Srivastava,PN
通过涉及羟基磷灰石、Sephadex G-200 和伴刀豆球蛋白 A-Sepharose 层析的三步程序，从兔精浆中纯化 β-N-乙酰己糖胺酶。纯化后的酶比活性为56μmol/min/mg蛋白质，纯化次数为226倍，酶活得率为54%。纯化的酶是电泳均质的。均质酶在 pH4.0 时表现出最佳活性。表观Km值和Vmax。每毫克蛋白质分别为 1.4 mM 和 56mu mol/min。 Ag+、Hg2+等金属离子和对氯汞苯甲酸强烈抑制酶活性。用 Beta-N-乙酰己糖胺酶和芳基硫酸酯酶 A 的混合物处理兔卵会导致透明带肿胀。
Beta-N-Acetylhexosaminidase was purified from the rabbit seminal plasma by a three-step procedure involving hydroxyapatite, Sephadex G-200 and concanavalin A–Sepharose chromatography. The specific activity of the purified preparation was 56mu mol/min per mg of protein, which represented a 226-fold purification and a 54% yield of the enzyme activity. The purified enzyme was electrophoretically homogeneous. The homogeneous enzyme showed optimal activity at pH4.0. The apparent Km value and Vmax. were 1.4 mM and 56mu mol/min per mg of protein respectively. Metal ions such as Ag + and Hg2+ and p-chloromercuribenzoate strongly inhibited the enzyme activity. The treatment of rabbit ova with a mixture of Beta-N-acetylhexosaminidase and arylsulphatase A results in the swelling of the zona pellucida.