Transcription factors in eukaryotic cells can functionally regulate gene expression by acting in oligomeric assemblies formed from an intrinsically disordered protein phase transition enabled by molecular crowding.
Transcription factors in eukaryotic cells can functionally regulate gene expression by acting in oligomeric assemblies formed from an intrinsically disordered protein phase transition enabled by molecular crowding.
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DOI：
10.1080/21541264.2018.1475806
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Leake MC
中科院分区：
文献类型：
--
作者：
Leake MC
体内高速单分子荧光显微镜表明，真核生物中的转录因子可以在分子拥挤和本质无序蛋白质介导的寡聚簇中发挥作用。这一发现解决了转录因子如何在复杂、异质的细胞环境中如此有效地找到其基因靶标的长期难题。缩写 CDF——累积分布函数； FRAP——光漂白后的荧光恢复； GFP——绿色荧光蛋白； STORM——随机光学重建显微镜； TF——转录因子； YFP - 黄色荧光蛋白
High-speed single-molecule fluorescence microscopy in vivo shows that transcription factors in eukaryotes can act in oligomeric clusters mediated by molecular crowding and intrinsically disordered protein. This finding impacts on the longstanding puzzle of how transcription factors find their gene targets so efficiently in the complex, heterogeneous environment of the cell. Abbreviations CDF - cumulative distribution function; FRAP - fluorescence recovery after photobleaching; GFP - Green fluorescent protein; STORM - stochastic optical reconstruction microscopy; TF - Transcription factor; YFP - Yellow fluorescent protein