ZIF-8-Assisted NaYF4:Yb,Tm@ZnO Converter with Exonuclease III-Powered DNA Walker for Near-Infrared Light Responsive Biosensor
ZIF-8-Assisted NaYF4:Yb,Tm@ZnO Converter with Exonuclease III-Powered DNA Walker for Near-Infrared Light Responsive Biosensor
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ZIF-8 辅助的 NaYF4:Yb,Tm@ZnO 转换器，具有核酸外切酶 III 驱动的 DNA 步行器，用于近红外光响应生物传感器
DOI：
10.1021/acs.analchem.9b04710
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发表时间：
2020-01-07
影响因子：
7.4
通讯作者：
Tang, Dianping
中科院分区：
文献类型：
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作者：
Lv, Shuzhen;Zhang, Kangyao;Tang, Dianping
这项工作报告了ZIF-8（ZIF：Zeolitic Imidazy框架）辅助NAYF4：YB，TM@Zno UpConverter，用于在近交易（NIR（NIR）放射效率下，对carcinoembryonic抗原（CEA）的光电化学（PEC）生物并构建了对HOMEMADE 3DDNA PRINTER的INTRADIDIATION cARCINOEMBRYONIC（CEA）。复合光敏材料NAYF4：YB，TM@Zno作为转换器以将NIR导入到光电流输出中，是从退火Nayf4：YB，TM@ZIF-8驱动的。 YB3+和TM3+ - 编码的NAYF4（NAYF4：YB，TM）将NIR激发转换为UV发射，与ZnO的吸收相匹配，以产生光电流。在目标CEA引入时，DNA Walker的摆动臂包括CEA适体的序列，在G-Rich-GICH锚定DNA轨道官能化的磁珠上进行了夹杂的生物组装，并用CEA捕获适体。此后，触发了DNA Walker，并根据部分互补的配对和外核酸酶III（EXO III）（EXO III）捕获了摇摆臂DNA，并通过燃烧的桥梁机构消耗了锚定DNA，以进入下一个周期。 DNA Walker的释放的鸟嘌呤（G）底座增强了由检测单元，深色盒和光平台组成的微型自制3D打印设备上的光电流响应。在最佳条件下，NAYF4：YB，TM@基于NIR的Light-Light-light驱动的PEC生物传感器具有高灵敏度和CEA传感的选择性，检测极限为0.032 ng ml（-1）。重要的是，我们的策略为开发基于NIR的PEC生物传感器的开发提供了新的地平线，该方面在开发了MOF衍生的光电材料，3D打印设备的灵活设计以及有效的信号放大模式的方面。
This work reports a ZIF-8 (ZIF: Zeolitic Imidazolate Framework)-assisted NaYF4:Yb,Tm@ZnO upconverter for the photoelectrochemical (PEC) biosensing of carcinoembryonic antigen (CEA) under near-infrared (NIR) irradiation on a homemade 3D-printed device with DNA walker-based amplification strategy. The composite photosensitive material NaYF4:Yb,Tm@ZnO, as converter to transfer NIR import to photocurrent output, was driven from annealed NaYF4:Yb,Tm@ZIF-8. Yb3+ and Tm3+-codoped NaYF4 (NaYF4:Yb,Tm) converted NIR excitation into UV emission, matching with the absorption of ZnO for in situ excitation to generate the photocurrent. Upon target CEA introduction, the swing arm of DNA walker including the sequence of CEA aptamer carried out the sandwiched bioassembly with CEA capture aptamer on the G-rich anchorage DNA tracks-functionalized magnetic beads. Thereafter, DNA walker was triggered, and the swing arm DNA was captured by the G-rich anchorage DNA according to partly complementary pairing and Exonuclease III (Exo III) consumed anchorage DNA by a burnt-bridge mechanism to go into the next cycle. The released guanine (G) bases from DNA walker enhanced the photocurrent response on a miniature homemade 3D-printed device consisting of the detection cell, dark box, and light platform. Under optimal conditions, NaYF4:Yb,Tm@ZnO-based NIR light-driven PEC biosensor presented high sensitivity and selectivity for CEA sensing with a detection limit of 0.032 ng mL(-1). Importantly, our strategy provides a new horizon for the development of NIR-based PEC biosensors in the aspect of developing MOF-derived photoelectric materials, flexible design of a 3D-printed device, and effective signal amplification mode.