Establishing an Accurate and Sensitive In Vitro Drug Screening System for Human Adenovirus Infection with Human Corneal Cells
Establishing an Accurate and Sensitive In Vitro Drug Screening System for Human Adenovirus Infection with Human Corneal Cells
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建立准确灵敏的人角膜细胞感染人腺病毒体外药物筛选系统
DOI：
10.1016/j.virol.2023.02.005
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发表时间：
2023
期刊：
影响因子：
3.7
通讯作者：
Eiich N Kodama.
中科院分区：
文献类型：
--
作者：
Mina Sasano;Hironori Hayashi;Kumi Kawaji;Emiko Usui;Eiich N Kodama.
流行性角膜结膜炎（EKC）是一种危险的高度传染性疾病，有可能在医院和其他社区环境中引起流行病暴发。人类腺病毒（hav）是EKC的病原体，目前尚无批准的药物用于治疗。为了建立一种新的眼部hav感染药物筛选系统，我们使用了一种非癌性但永生化的人角膜上皮细胞系CRL11516。Brincidoforvir和3′-脱氧-3′-氟胸苷对hav C型1 （C1）、C2、E4和C6的复制抑制程度相同。这种替代检测系统可以在2天内评估化合物的抗hav活性和细胞毒性，而不需要兔眼感染模型。
Epidemic keratoconjunctivitis (EKC) is a hazardous and highly contagious disease, with the potential to cause epidemic outbreaks in hospitals and other community settings. There are currently no approved drugs for human adenovirus (HAdV), the causative agent of EKC. To establish a novel drug screening system for ocular HAdV infections, we employed CRL11516, a non-cancerous but immortalized human corneal epithelial cell line. Brincidoforvir and 3′-deoxy-3′-fluorothymidine inhibit replication of HAdV species C type 1 (C1), C2, E4, and C6 to the same extent. This alternative assay system may allow for the evaluation of anti-HAdV activity and cell cytotoxicity of compounds within 2 days and without the need of the rabbit eye infection model.