Genetic identification of functions of TR‐DNA transcripts in octopine crown galls
Genetic identification of functions of TR‐DNA transcripts in octopine crown galls
复制标题
章鱼冠瘿TR-DNA转录本功能的遗传鉴定
DOI：
10.1002/j.1460-2075.1984.tb01774.x
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
J. Schell
中科院分区：
文献类型：
--
作者：
F. Salomon;R. Deblaere;J. Leemans;J. Hernalsteens;M. Van Montagu;J. Schell
在农杆菌章鱼碱 Ti 质粒 B6S3 的 TR 区构建了 17 个体内和体外插入突变。它们包括五个 TR 转录本中每一个的突变。所有突变体均保留其致癌特性并诱导具有野生型形态的肿瘤。对突变农杆菌菌株诱导的肿瘤中的观点的测定表明，甘露碱和农架碱的产生是 TR 相关性状。两个转录本对于合成甘露碱是必需的，第三个转录本对于将甘露碱转化为农架碱是必需的。所有突变都不会阻止意见的排泄。此外，五个转录本中没有一个对于 TR 区的转移和整合是必需的。
Seventeen in vivo and in vitro insertion mutations in the TR‐region of the octopine Ti plasmid B6S3 of Agrobacterium tumefaciens were constructed. They include mutations in each of the five TR transcripts. All mutants retained their oncogenic properties and induced tumors with wild‐type morphology. Determination of opines in tumors induced by mutant Agrobacterium strains showed that production of mannopine and agropine are TR‐linked traits. Two transcripts are necessary for the synthesis of mannopine and a third one for the conversion of mannopine into agropine. None of the mutations prevents the excretion of opines. In addition, none of the five transcripts is essential for transfer and integration of the TR‐region.