Gel filtration: a new method to analyze and separate red blood cells with different deformabilities.
Gel filtration: a new method to analyze and separate red blood cells with different deformabilities.
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凝胶过滤：一种分析和分离不同变形能力红细胞的新方法。
DOI：
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发表时间：
1990
期刊：
影响因子：
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通讯作者：
P. Straub
中科院分区：
文献类型：
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作者：
F. Rohner;W. Reinhart;A. Haeberli;P. Straub
通过琼脂糖凝胶（Sephadex、Sepharose和Superose）过滤红细胞用于评估红细胞变形性，同时获得具有不同性质的红细胞级分。使用每个级分中的血红蛋白浓度来定义红细胞的过滤曲线。发现凝胶过滤是一个可重复的过程。凝胶过滤指数（GFI）由过滤曲线在最大高度一半处的高度和宽度推导得出。使用渗透处理红细胞、戊二醛浓度增加时部分硬化的红细胞以及正常红细胞和硬化红细胞的混合物检测该方法。红细胞变形能力的变化灵敏地检测GFI。从柱中洗脱的第一部分含有具有正常变形能力的红细胞，而后面的部分主要含有变形性较小的细胞，其膜弹性模量增加。通过对后期组分的重复过滤，可以用较少变形的细胞富集悬浮液。慢性肾功能衰竭患者的滤过率降低;他们的GFI为1.04 +/- 0.27（n = 15），而对照组为1.18 +/- 0.17（n = 20，p <0.05）。我们的结论是，凝胶过滤是有用的检测和分离异常红细胞亚群。它可能是一个有价值的工具，调查疾病，如镰状细胞贫血。
Filtration of red blood cells through agarose gels (Sephadex, Sepharose, and Superose) was used to assess red cell deformability and simultaneously obtain fractions of red cells with different properties. The hemoglobin concentration in each fraction was used to define a filtration profile of red cells. It was found that gel filtration was a reproducible process. A gel filtration index (GFI) was derived from the height and the width of the filtration curve at half the maximum height. Osmotically treated red cells, red cells partially hardened with increasing glutaraldehyde concentrations, and mixtures of normal and hardened red cells were used to test the method. Changes in red cell deformability were sensitively detected by the GFI. The first fractions eluted from the column contained red cells with normal deformability, whereas the later fractions contained primarily less deformable cells with an increased membrane elastic modulus. By repeated filtration of late fractions it was possible to enrich the suspensions with less deformable cells. Patients with chronic renal failure had a decreased filterability; their GFI was 1.04 +/- 0.27 (n = 15) compared with 1.18 +/- 0.17 in controls (n = 20, p less than 0.05). We conclude that gel filtration is useful to detect and isolate abnormal red cell subpopulations. It could be a valuable tool for the investigation of disorders such as sickle cell anemia.