Determining antioxidant activities of lactobacilli by cellular antioxidant assay in mammal cells
Determining antioxidant activities of lactobacilli by cellular antioxidant assay in mammal cells
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通过哺乳动物细胞中的细胞抗氧化测定测定乳酸杆菌的抗氧化活性
DOI：
10.1016/j.jff.2015.09.017
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发表时间：
2015-12-01
影响因子：
5.6
通讯作者：
Chen, Wei
中科院分区：
文献类型：
--
作者：
Xing, Jiali;Wang, Gang;Chen, Wei
在乳杆菌属的有益效果中，抗氧化活性被认为是保护健康的共同机制。鼠李属L.嗜酸乳杆菌L. casei和L. fermenti）进行测定。比较了不同细胞系中CFS和SS的相关性，以及三种细胞模型（RAW264.7、Caco-2和EA.hy926细胞系）之间的相关性。乳酸杆菌的所有CFSs和SSs显示，它们显着抑制细胞内活性氧的产生，而不产生明显的细胞毒性作用在三种哺乳动物细胞系。这些结果表明，RAW264.7，Caco-2，和EA.hy926细胞系可以作为细胞模型来测定CFS和SS的乳酸杆菌的细胞抗氧化活性。因此，乳酸杆菌及其副产物是天然抗氧化剂和减少氧化应激的潜在来源。(C)2015爱思唯尔有限公司版权所有。
Among the beneficial effects of Lactobacillus spp., the common mechanism underlying health protection is believed to be related to antioxidant activity The antioxidant activities of cell-free suspension (CFS) and strain suspension (SS) of 13 Lactobacillus strains derived from 4 species (L. rhamnosus, L. acidophilus, L. casei, and L. fermenti) were determined. The correlation of CFSs and SSs in different cell lines, as well as the correlation among the three cell models (RAW264.7, Caco-2, and EA.hy926 cell lines), were compared. All CFSs and SSs of lactobacilli showed that they significantly inhibited the production of intracellular reactive oxygen species without creating obvious cytotoxic effects in the three mammal cell lines. These results suggested that RAW264.7, Caco-2, and EA.hy926 cell lines could be used as cell models to assay the cellular antioxidant activity of CFS and SS of lactobacilli. Thus, lactobacilli and by-products are a potential source of natural antioxidants and to reduce oxidative stress. (C) 2015 Elsevier Ltd. All rights reserved.