Detection of the two short tandem repeat loci (HumTPO and HumLPL) in Japanese populations using discontinuous polyacrylamide gel electrophoresis.
Detection of the two short tandem repeat loci (HumTPO and HumLPL) in Japanese populations using discontinuous polyacrylamide gel electrophoresis.
复制标题
使用不连续聚丙烯酰胺凝胶电泳检测日本人群中的两个短串联重复基因座（HumTPO 和 HumLPL）。
DOI：
10.1520/jfs14043j
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
K. Kishi
中科院分区：
文献类型：
--
作者：
H. Takeshita;T. Yasuda;T. Nakajima;O. Hosomi;Y. Nakashima;S. Tsutsumi;K. Kishi
采用不连续水平聚丙烯酰胺凝胶电泳和银染技术，对居住在日本群马县和福井县的218名无关日本人的两种短串联重复序列（STR）系统HumTPO和HumLPL进行了研究。在HumTPO和HumLPL基因座分别鉴定出5个和6个等位基因。没有偏离Hardy-Weinberg平衡或基因型频率的差异，发现在两个系统中的两个日本人群之间。我们的检测方法提供了几个优点，包括节省时间和安全的电泳程序，和快速和容易的银染，与标准和传统的STR分析使用垂直聚丙烯酰胺凝胶电泳。
Two short tandem repeat (STR) systems, HumTPO and HumLPL, were investigated in 218 unrelated Japanese living in Gunma and Fukui Prefectures by means of discontinuous, horizontal polyacrylamide gel electrophoresis and silver staining. Five and six alleles were identified at the HumTPO and HumLPL loci, respectively. No deviations from the Hardy-Weinberg equilibrium or differences in genotype frequency were found between the two Japanese populations in the two systems. Our detection methods offered several advantages including time-saving and safe electrophoretic procedures, and fast and easy silver-staining, in comparison with standard and conventional STR analysis using vertical polyacrylamide gel electrophoresis.