Store-operated calcium entry promotes the degradation of the transcription factor Sp4 in resting neurons.
Store-operated calcium entry promotes the degradation of the transcription factor Sp4 in resting neurons.
复制标题
DOI：
10.1126/scisignal.2005242
复制
发表时间：
2014-06-03
影响因子：
7.3
通讯作者：
Gill G
中科院分区：
文献类型：
--
作者：
Lalonde J;Saia G;Gill G
Calcium (Ca2+) signaling activated in response to membrane depolarization regulates neuronal maturation, connectivity and plasticity, but the role of store-operated Ca2+ entry (SOCE) in neurons is poorly understood. Here, we report that SOCE is regulated by membrane potential in cerebellar granule neurons (CGNs) in the opposite direction of voltage-gated Ca2+ channels; maximal activation of SOCE occurs in resting conditions where this Ca2+ influx promotes the degradation of transcription factor Sp4, a regulator of neuronal morphogenesis and function. We report that lowering [K+]EXT caused depletion of intracellular Ca2+ stores and induced a Ca2+ influx with characteristics of SOCE. Under these conditions, we also observed the relocalization of the ER Ca2+ sensor STIM1. We found that compounds that block SOCE prevented the ubiquitination and degradation of Sp4 in low extracellular potassium ([K+]EXT). Further, we show that STIM1 knockdown blocked degradation of Sp4 while expression of a constitutively active STIM1 decreased Sp4 protein abundance under depolarization conditions. Our findings provide evidence for the dynamic regulation and downstream signaling effect of SOCE in neurons and suggest a new pathway for Ca2+ signaling to shape neuronal gene expression.
登录
查看更多内容
影响因子：
5.3
作者：
Berna-Erro A;Woodard GE;Rosado JA
通讯作者：
Rosado JA
影响因子：
16.2
作者：
Flavell, Steven W.;Kim, Tae-Kyung;Gray, Jesse M.;Harmin, David A.;Hemberg, Martin;Hong, Elizabeth J.;Markenscoff-Papadimitriou, Eirene;Bear, Daniel M.;Greenberg, Michael E.
通讯作者：
Greenberg, Michael E.
DOI：
10.1523/jneurosci.5053-10.2011
发表时间：
2011-03-09
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Gemes G;Bangaru ML;Wu HE;Tang Q;Weihrauch D;Koopmeiners AS;Cruikshank JM;Kwok WM;Hogan QH
通讯作者：
Hogan QH
影响因子：
4.8
作者：
Darbellay, Basile;Arnaudeau, Serge;Bernheim, Laurent
通讯作者：
Bernheim, Laurent
影响因子：
64.8
作者：
HOTH, M;PENNER, R
通讯作者：
PENNER, R