Finding functional features in Saccharomyces genomes by phylogenetic footprinting
Finding functional features in Saccharomyces genomes by phylogenetic footprinting
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DOI：
10.1126/science.1084337
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发表时间：
2003-07-04
期刊：
影响因子：
56.9
通讯作者：
Johnston, M
中科院分区：
文献类型：
--
作者：
Cliften, P;Sudarsanam, P;Johnston, M
在进化过程中发生的DNA序列筛选和筛选导致非功能序列分化，在基因组序列比较中留下功能序列元素的系统发育足迹。我们在6种酵母菌的基因组序列中寻找了这样的足迹，并鉴定了潜在的功能序列。这些序列的比较使我们能够修改酵母基因目录，并确定可能是转录调节蛋白靶点的序列基序。其中一些保守序列基序位于具有相似功能注释或相似表达模式的基因的上游，或由相同转录因子结合的基因，因此是功能调控序列的良好候选者。
The sifting and winnowing of DNA sequence that occur during evolution cause nonfunctional sequences to diverge, leaving phylogenetic footprints of functional sequence elements in comparisons of genome sequences. We searched for such footprints among the genome sequences of six Saccharomyces species and identified potentially functional sequences. Comparison of these sequences allowed us to revise the catalog of yeast genes and identify sequence motifs that may be targets of transcriptional regulatory proteins. Some of these conserved sequence motifs reside upstream of genes with similar functional annotations or similar expression patterns or those bound by the same transcription factor and are thus good candidates for functional regulatory sequences.