Simvastatin and vitamin E effects on cardiac and hepatic oxidative stress in rats fed on high fat diet
Simvastatin and vitamin E effects on cardiac and hepatic oxidative stress in rats fed on high fat diet
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DOI：
10.1007/s13105-013-0250-y
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发表时间：
2013-12-01
影响因子：
3.4
通讯作者：
Sakr, Hussein F.
中科院分区：
文献类型：
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作者：
Abbas, Amr M.;Sakr, Hussein F.
高脂饮食是代谢综合征和2型糖尿病的常见病因。已发表的数据显示，HFD和随后的血脂异常是氧化应激的主要触发因素。48只雄性Sprague-Dawley大鼠，体重170-200 g，分为6组：对照组、维生素E对照组（100 mg/kg/天，腹腔注射）、辛伐他汀（SIM）对照（10 mg/kg体重/天）、HFD、含维生素E的HFD和含SIM的HFD。给予标准和高胆固醇饮食15周，并在最后4周添加SIM和维生素E。在所有大鼠中，血清维生素E、总胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白（LDL）和高密度脂蛋白（HDL）、丙氨酸（ALT）和天冬氨酸（AST）转氨酶、碱性磷酸酶（ALP）和γ谷氨酰转肽酶（GGT）以及心脏和肝脏硫代巴比妥酸反应物质（TBARS）和抗氧化剂（还原型谷胱甘肽（GSH）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT））。此外，还记录了心电图（ECG）。HFD可显著增加QTc间期、心率（HR）、血清TC、TG、LDL、ALT、AST、ALP、GGT、肝脏TG、心脏和肝脏TBARS，但降低抗氧化剂和HDL，而SIM可降低HR、肝脏TG、血清TC、TG和LDL，升高HDL。维生素E没有效果。此外，SIM和维生素E降低QTc间期，血清ALT，AST，ALP，GGT，心脏和肝脏TBARS和增加抗氧化剂在HFD大鼠。组织病理学观察证实了生化参数。SIM和维生素E减缓高胆固醇血症诱导的肝脏和心脏氧化应激的进展，并改善其功能。
High fat diet (HFD) is a common cause of metabolic syndrome and type 2 diabetes mellitus. Published data showed that HFD and subsequent dyslipidemia are major triggers for oxidative stress. Forty-eight male Sprague-Dawley rats, weighing 170-200 g, were divided into six groups: control, control with vitamin E (100 mg/kg/day, i.p.), control with simvastatin (SIM) (10 mg/kg of body weight/day), HFD, HFD with vitamin E, and HFD with SIM. Standard and high cholesterol diets were given for 15 weeks and SIM and vitamin E were added in the last 4 weeks. In all rats, serum vitamin E, total cholesterol (TC), triglycerides (TG), low (LDL) and high (HDL) density lipoproteins, alanine (ALT) and aspartate (AST) transaminases, alkaline phosphatase (ALP), and gamma glutamyl transpeptidase (GGT) as well as cardiac and hepatic thiobarbituric acid-reactive substances (TBARS) and antioxidants (reduced glutathione (GSH), superoxide dismutase (SOD), and catalase (CAT)) were measured. Also, electrocardiogram (ECG) was recorded. HFD significantly increased QTc interval, heart rate (HR), serum TC, TG, LDL, ALT, AST, ALP, GGT, liver TG, and cardiac and hepatic TBARS but decreased antioxidants and HDL, while SIM decreased HR, liver TG, serum TC, TG, and LDL and increased HDL in HFD rats. Vitamin E had no effect. Moreover, SIM and vitamin E decreased QTc interval, serum ALT, AST, ALP, GGT, and cardiac and hepatic TBARS and increased antioxidants in HFD rats. Histopathological observations confirm the biochemical parameters. SIM and vitamin E slow progression of hypercholesterolemia-induced oxidative stress in liver and heart and improve their functions.