Murine maternal cell microchimerism: analysis using real-time PCR and in vivo imaging.
Murine maternal cell microchimerism: analysis using real-time PCR and in vivo imaging.
复制标题
小鼠母体细胞微嵌合：使用实时 PCR 和体内成像进行分析。
DOI：
10.1095/biolreprod.107.063305
复制
发表时间：
2008
影响因子：
3.6
通讯作者：
Bianchi,DianaW
中科院分区：
文献类型：
--
作者：
Su,EricC;Johnson,KirbyL;Tighiouart,Hocine;Bianchi,DianaW
在人类中，母体细胞存在于患有炎性肌病、硬皮病和新生儿狼疮的儿童的受影响组织中。母体细胞微嵌合体（MCM）是否与疾病的病理学有关尚不清楚。我们试图了解影响MCM的因素，作为未来机制研究的基线。使用小鼠模型，我们培育了野生型（WT）雄性转基因荧光素酶（Luc）报告基因的雌性小鼠。在不同的出生后年龄处死WT后代。从多个器官中提取DNA，并使用实时PCR扩增来定量Luctransgenic作为母源性细胞的标记。灵敏度为每100 000个WT细胞中有1 - 2个转基因细胞。 在85%的小鼠和45%的组织中观察到MCM。平均每10万个新生儿细胞中有158个母细胞。 MCM出现频率和数量最高的器官是心和肺（P< 0.001）。出生后21天的年龄似乎没有影响MCM水平（P= 0.47），而增加奇偶校验可能会增加MCM水平。数据显示MCM在健康新生小鼠中是常见的，其存在于它们的主要器官中，并且存在器官特异性差异。这可能代表母体细胞的差异迁移或特定胎儿器官对微嵌合体的不同接受性。妊娠史似乎在母体细胞贩运中发挥作用。MCM在妊娠和疾病发病机制中的作用仍有待阐明。
In humans, maternal cells are present in the affected tissues of children with inflammatory myopathy, scleroderma, and neonatal lupus. It is unknown if maternal cell microchimerism (MCM) contributes to the pathology of disease. We sought to understand the factors that affect MCM to serve as a baseline for future mechanistic studies. Using a mouse model, we bred female mice transgenic for the luciferase (Luc) reporter gene to wild-type (WT) males. The WT offspring were sacrificed at various postnatal ages. DNA was extracted from multiple organs, and real-time PCR amplification was used to quantifyLuctransgene as a marker for maternally derived cells. Sensitivity was one to two transgenic cells per 100 000 WT cells. MCM was noted in 85% of mice and 45% of tissues assayed. The average quantity of MCM was 158 maternal cells per 100 000 neonatal cells. The organs displaying the highest frequency and quantity of MCM were heart and lung (P< 0.001). Postnatal age up to 21 days did not appear to affect levels of MCM (P= 0.47), whereas increasing parity may increase levels of MCM. The data show that MCM is a common occurrence in healthy newborn mice, that it is present in their major organs, and that there are organ specific differences. This may represent differential migration of maternal cells or varying receptivity of specific fetal organs to microchimerism. Pregnancy history appears to play a role in maternal cell trafficking. The role of MCM in pregnancy and disease pathogenesis remains to be elucidated.