Infection of aquatic insects with trematode metacercariae carrying Ehrlichia risticii, the cause of Potomac horse fever
Infection of aquatic insects with trematode metacercariae carrying Ehrlichia risticii, the cause of Potomac horse fever
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DOI：
10.1603/0022-2585-37.4.619
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发表时间：
2000-07-01
影响因子：
2.1
通讯作者：
Madigan, JE
中科院分区：
文献类型：
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作者：
Chae, JS;Pusterla, N;Madigan, JE
我们利用巢式聚合酶链反应（PCR）扩增以及16S rRNA、51 kDa主要抗原和groEL热休克蛋白基因的序列分析，在从加利福尼亚北部一个牧场溪流中采集的水生昆虫体内发现的吸虫阶段中找到了立氏埃立克体（波托马克马热的病原体）存在的证据。在以下昆虫的幼虫和成虫体内发现的囊蚴中检测到了立氏埃立克体：毛翅目昆虫（石蛾）、蜉蝣目昆虫（蜉蝣）、蜻蜓目昆虫（束翅亚目，豆娘）、蜻蜓目昆虫（差翅亚目，蜻蜓）以及襀翅目昆虫（石蝇）。立氏埃立克体在水生昆虫中的流行率为31.9%（n = 454个个体）（17个物种中有13个呈阳性）。各目的流行率如下：毛翅目昆虫中为43.5%（n = 207），蜉蝣目昆虫中为15.2%（n = 92），蜻蜓目昆虫（束翅亚目）中为13.9%（n = 115），蜻蜓目昆虫（差翅亚目）中为10.0%（n = 10），襀翅目昆虫中为80.0%（n = 30）。这项研究表明，作为立氏埃立克体传播媒介的吸虫具有广泛的中间宿主范围。昆虫可能在这种疾病的流行病学中发挥重要作用。
We provide evidence of Ehrlichia risticii Holland, the agent of Potomac horse fever, in trematode stages found in aquatic insects collected from a pasture stream in northern California, using nested polymerase chain reaction (PCR) amplification and sequence analyses of the 16S rRNA, 51 kDa major antigen and groEL heat shock protein genes. E, risticii was detected in metacercariae found in the immatures and adults of the following insects: caddisflies (Trichoptera), mayflies (Ephemeroptera), damselflies (Odonata, Zygoptera), dragonflies (Odonata, Anisoptera),and stoneflies (Plecoptera). The prevalence of E, risticii was 31.9% (n = 454 individuals) in aquatic insects (13 of 17 species were positive). Prevalence within orders was as follows: 43.5% (n = 207) in caddisflies, 15.2% (n = 92) in mayflies, 13.9% (n = 115) in damselflies, 10.0% (n = 10) in dragonflies, and 80.0% (n = 30) in stoneflies. This study demonstrates a broad intermediate host range for trematodes that act as vector for E. risticii. Insects are likely to play an important role in the epidemiology of this disease.