Allelic polymorphism of MSP2 gene in severe P-falciparum malaria in an area of low and seasonal transmission
Allelic polymorphism of MSP2 gene in severe P-falciparum malaria in an area of low and seasonal transmission
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DOI：
10.1007/s00436-007-0716-3
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发表时间：
2007-12-01
影响因子：
2
通讯作者：
Giha, Hayder A.
中科院分区：
文献类型：
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作者：
A-Elbasit, Ishraga E.;ElGhazali, Gehad;Giha, Hayder A.
预计重症疟疾(SM)和无并发症疟疾(UM)感染具有不同的基因构成。在这项研究中，来自苏丹东部的325名患有SM和UM的献血者和未患疟疾的献血者(包括无症状的亚显微疟疾-Asum)采集了血液样本。SM组包括脑型疟疾(CM)、严重疟疾贫血(SMA)和其他并发症患者。利用MSP2基因座进行寄生虫基因分型。我们发现该寄生虫种群的遗传多样性显著(51种基因类型)。总感染复数(MOI)为1.5，SM与UM相当。然而，ASM(1.0)和致命性CM(1.14)的MOI与UM(1.53)、SMA(1.52)和非致命性CM(1.7)相似且显著低于UM(1.53)、SMA(1.52)和非致命性CM(1.7)。等位基因Ic1和Fc27在SM和UM中的比例相当，且等位基因大小的分布具有相关性(相关系数分别为0.59和0.718；P<；0.001)。值得注意的是，FC27基因在ASM(68.2%)中过度表达，在致死性CM中未被识别，而在混合克隆性感染中，奎宁治疗后IC1的清除速度快于FC27的清除。最后，多克隆感染(IC1和FC27)的组成表明MSP2基因家族内部而不是之间存在更强的交叉免疫。
The severe malaria (SM) and uncomplicated malaria (UM) infections are expected to have different genetic makeup. In this study, blood samples were obtained from 325 donors with SM and UM and malaria-free donors (including asymptomatic submicroscopic malaria-ASUM), from Eastern Sudan. The SM group included patients with cerebral malaria (CM), severe malarial anemia (SMA), and other complications. The MSP2 locus was exploited for parasite genotyping. We found that the genetic diversity of the parasite population was marked (51 genotypes). The overall multiplicity of infection (MOI) was 1.5, and it was comparable between SM and UM. However, the MOI in ASUM (1.0) and fatal CM (1.14) was comparable and significantly lower than in UM (1.53), SMA (1.52), and nonfatal CM (1.7). The ratio of the IC1 to FC27 allele families was comparable between SM and UM, and the distribution of the allele sizes was correlated (correlation coefficient=0.59 and 0.718; P < 0.001). It is interesting to note that the FC27 genotype was overrepresented in ASUM (68.2%) and was not recognized in fatal CM, while in mixed-clone infections, the clearance of IC1 after quinine treatment was faster than FC27 clearance. Finally, the composition of the multiclone infections (IC1 and FC27) was suggesting a stronger cross-immunity within rather than between MSP2 gene families.