BIOTECHNOLOGY CORE FACILITIES - TRENDS AND UPDATE
BIOTECHNOLOGY CORE FACILITIES - TRENDS AND UPDATE
复制标题
DOI：
10.1096/fasebj.7.12.8375609
复制
发表时间：
1993-09-01
期刊：
影响因子：
4.8
通讯作者：
HAYES, TK
中科院分区：
文献类型：
--
作者：
IVANETICH, KM;NIECE, RL;HAYES, TK
对128个生物技术核心设施的调查提供了关于财务、服务、空间需求和人员的数据。平均每个设施有四名全职人员和7.5个主要仪器系统，占地969平方米。英尺平均总收入为244 000美元/年，但年度用户费收入仅为125 000美元。这些设施通常需要大量的机构支助或赠款。成本回收（用户费收入除以总收入）平均为49%。在过去5年中，用户费收入、总收入和成本回收都有所增加。蛋白质测序和肽合成的内部费用增加了约30%，而寡核苷酸合成费用减少了74%。大多数服务的成本（对非使用者费用收入的补贴进行了校正）没有显著变化，但寡核苷酸合成成本在1992年下降了25%。DNA合成每月的处理量最高（116个样品），其次是氨基酸分析（86个样品）和DNA测序（67个样品）。其他服务平均抽样5至60个。DNA合成和纯化是最多的主要研究者使用的服务。在过去的3年里，已经推出了许多服务，包括DNA测序、质谱、毛细管电泳、RNA合成、电印迹和碳水化合物分析。虽然这些服务的特点是方法开发和非用户运行水平高，但提供这些服务的设施所占百分比是1989年的两倍，而且对设施收入的贡献越来越大。
A survey of 128 biotechnology core facilities has provided data on the finances, services, space requirements, and personnel. An average facility had four full-time personnel and 7.5 major instrument systems, and occupied 969 sq. ft. Average total income was $244,000/year, but annual user fee income was only $125,000. Typically, facilities required substantial institutional support or grants. Cost recovery (user fee income divided by total income) averaged 49%. During the last 5 years user fee income, total income, and cost recovery have increased. In-house charges for protein sequencing and peptide synthesis increased approximately 30%, while oligonucleotide synthesis charges decreased by 74%. The costs (charges corrected for subsidy from non-user fee income) for most services did not significantly change, except that oligonucleotide synthesis costs decreased by 25% in 1992. DNA synthesis had the highest throughput per month (116 samples), followed by amino acid analysis (86 samples) and DNA sequencing (67 samples). Other services averaged from 5 to 60 samples. DNA synthesis and purification were the services used by the greatest number of principal investigators. A number of services including DNA sequencing, mass spectrometry, capillary electrophoresis, RNA synthesis, electroblotting, and carbohydrate analysis have been introduced in the last 3 years. Although these services are characterized by high levels of methods development and non-user runs, they are offered by twice the percentage of facilities as in 1989, and are increasingly contributing to facility income.