Peanut allergen induces more serious allergic reactions than other allergens involving MAPK signaling pathways.
Peanut allergen induces more serious allergic reactions than other allergens involving MAPK signaling pathways.
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DOI：
10.1039/d2fo00777k
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发表时间：
2022-08
期刊：
影响因子：
6.1
通讯作者：
Junjuan Wang;Ruolin Song;R. Lan;Mengzhen Hao;Guirong Liu;Manman Liu;Shanfeng Sun;Cheng Chen;H. Che
中科院分区：
文献类型：
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作者：
Junjuan Wang;Ruolin Song;R. Lan;Mengzhen Hao;Guirong Liu;Manman Liu;Shanfeng Sun;Cheng Chen;H. Che
对不同食物过敏原引发的过敏反应的严重程度进行分类，目前还没有普遍接受的统一研究。我们建立了一个食物过敏模型，该模型基于反复灌胃花生、鸡蛋、牛奶或大豆蛋白与霍乱毒素混合，然后口服高剂量致敏蛋白。通过酶联免疫吸附试验、苏木精-伊红染色和甲苯胺蓝染色发现，过敏组的特异性IgE、特异性IgG1、过敏症状评分、组胺、小鼠肥大细胞蛋白酶-1、血管渗漏、Th2细胞因子、肺和肠肥大细胞浸润均升高。每个致敏组在口服食物刺激后体温和Th1细胞因子均下降。实时荧光定量PCR和western blotting检测结果显示，Th2细胞因子、IL-25、IL-33、TSLP水平升高，哮喘相关基因ARG1、DCN、LTB4R1、NFKBIA水平升高，MAPK信号通路激活。从食物过敏的严重程度来看，花生过敏最严重，其次是鸡蛋和牛奶，大豆过敏最不严重。与其他过敏组相比，花生过敏组哮喘基因通过MAPK信号通路调控产生相关Th2细胞因子；因此，花生组的老鼠表现出更严重的过敏反应。对食物过敏的严重程度进行比较是对严重食物过敏采取温和预防措施的必要条件。
There is no universally accepted uniform research to classify the severity of allergic reactions triggered by different food allergens. We established a food allergy model based on repeated intragastric administrations of proteins from peanut, egg, milk, or soybean mixed with cholera toxin followed by oral food challenges with a high dose of the sensitizing proteins. Increased specific IgE, specific IgG1, allergic symptom scores, histamine, murine mast cell proteases-1, vascular leakage, Th2 cytokines, and mast cell infiltration in the lungs and intestine were found in the allergic groups via enzyme-linked immunosorbent assay, hematoxylin-eosin, and toluidine blue staining. Each sensitized group showed a decrease in body temperature and Th1 cytokines after oral food challenge. The increased levels of Th2 cytokines, IL-25, IL-33, and TSLP, and related asthma genes ARG1, DCN, LTB4R1 and NFKBIA as well as the activation of MAPK signaling pathways were also revealed by quantitative real-time PCR and western blotting. In terms of the severity of food allergies, peanut allergy was the most serious followed by egg and milk, and soybean allergy was the least severe. Compared to other allergic groups, asthma genes were regulated through the MAPK signaling pathways to produce related Th2 cytokines in peanut allergy; consequently, mice in the peanut group exhibited more severe allergic reactions. Comparison of the severity of food allergies is required for the development of milder prevention for severe food allergies.