Isolation and characterization of novel polymorphic microsatellite loci for the deep-sea hydrothermal vent limpet, Lepetodrilus nux, and the vent-associated squat lobster, Shinkaia crosnieri
Isolation and characterization of novel polymorphic microsatellite loci for the deep-sea hydrothermal vent limpet, Lepetodrilus nux, and the vent-associated squat lobster, Shinkaia crosnieri
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深海热液喷口帽贝（Lepetodrilus nux）和喷口相关蹲龙虾（Shinkaia crosnieri）新型多态性微卫星位点的分离和表征
DOI：
10.1007/s12526-017-0704-5
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发表时间：
2017
影响因子：
1.6
通讯作者：
Mitarai Satoshi
中科院分区：
文献类型：
--
作者：
Nakajima Yuichi;Shinzato Chuya;Khalturina Mariia;Nakamura Masako;Watanabe Hiromi Kayama;Nakagawa Satoshi;Satoh Noriyuki;Mitarai Satoshi
最近的遗传学研究已开始揭示深海热液喷口物种的种群结构，但根据多个遗传位点对热液喷口种群的遗传多样性和连通性进行的详细评估仍然很少，特别是在西北太平洋。因此，我们分离出38个新的多态性微卫星位点的帽贝，Lepetodrilus nux，和14个从蹲龙虾，Shinkaia crosnieri，两个占主导地位的热液喷口物种，使用下一代测序。这些位点在L中显示了5-20个等位基因的多态性水平。克罗涅利链球菌中的nuxand 5-25。观察到的和预期的杂合性范围为0.240至0.960和0.283至0.938 inL。nuxand分别为0.450 ~ 0.950和0.620 ~ 0.941。在L. nux 4个位点均显著偏离Hardy-Weinberg平衡（p< 0.05）。本研究中的微卫星位点将有助于详细测量群体间的遗传多样性和连接性，并更好地了解L. nuxandS.在西北太平洋的深海群落中。
Recent genetic research has begun to reveal population structures of deep-sea, hydrothermal vent species, but detailed assessments of genetic diversity and connectivity in hydrothermal vent populations, based on multiple genetic loci, are still scarce, especially in the Northwest Pacific. Accordingly, we isolated 38 novel polymorphic microsatellite loci from the limpet,Lepetodrilus nux, and 14 from the squat lobster,Shinkaia crosnieri, two dominant hydrothermal vent species, using next-generation sequencing. These loci revealed polymorphism levels of 5–20 alleles per locus inL. nuxand 5–25 inS. crosnieri. Observed and expected heterozygosities ranged from 0.240 to 0.960 and 0.283 to 0.938 inL. nuxand from 0.450 to 0.950 and 0.620 to 0.941 inS. crosnieri, respectively. Twelve loci inL. nuxand four loci inS. crosnierishowed significant deviation from Hardy–Weinberg equilibrium (p< 0.05). Microsatellite loci evaluated in this study will enable detailed measurements of genetic diversity and connectivity among populations, and better understanding of evolutionary divergence processes ofL. nuxandS. crosnieriin deep-sea communities in the Northwest Pacific.