Plant-Mediated Synthesis of Au Nanoparticles: Separation and Identification of Active Biomolecule in the Water Extract of Cacumen Platycladi
Plant-Mediated Synthesis of Au Nanoparticles: Separation and Identification of Active Biomolecule in the Water Extract of Cacumen Platycladi
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植物介导金纳米粒子的合成：侧柏水提取物中活性生物分子的分离与鉴定
DOI：
10.1021/acs.iecr.7b00064
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发表时间：
2017-05-10
影响因子：
4.2
通讯作者：
Li, Qingbiao
中科院分区：
文献类型：
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作者：
Liu, Hai;Lian, Ting;Li, Qingbiao
为了研究活性分子在侧柏叶提取物仿生合成金纳米颗粒过程中的作用，采用快速柱色谱根据极性差异分离侧柏叶提取物。采用傅里叶变换红外光谱和配备光电二极管阵列检测器的高效液相色谱来鉴定分离样品的类别。结果表明，侧柏叶提取物中的成分可分为三类：糖类（Sloading和S0）、多酚（S1类似于S5）和类黄酮（S6类似于S8）。每个分离样品与氯金酸产生的金纳米颗粒的特征表明，还原能力从强到弱的顺序是多酚、类黄酮和糖。糖对金纳米粒子的生物合成没有保护作用，多酚参与各向同性稳定，而类黄酮则负责各向异性生长。
In order to investigate the roles of active molecules during the bioinspired synthesis process of gold nanoparticles by Cacumen platycladi leaf extract, flash column chromatography was employed to separate the Cacumen platycladi leaf extract according to the polarity differences. Fourier transform infrared spectroscopy and high-performance liquid chromatography equipped with a photodiode array detector were adopted to identify the category of the isolated samples. The results have demonstrated that components in the Cacumen platycladi leaf extract can be sorted into three categories: sugars (the Sloading and S0), polyphenols (S1 similar to S5), and flavonoids (S6 similar to S8). The characterizations of gold nanoparticles, produced by each isolated sample with chloroauric acid, indicate the strong-to-weak sequence of reducing power is polyphenols, flavonoids, and sugars. Sugars have no protection strength for the biosynthesis of gold nanoparticles, polyphenols involved in isotropous stabilization, while flavonoids are responsible for the anisotropic growth.