CADAVERINE IS COVALENTLY LINKED TO PEPTIDOGLYCAN IN SELENOMONAS-RUMINANTIUM
CADAVERINE IS COVALENTLY LINKED TO PEPTIDOGLYCAN IN SELENOMONAS-RUMINANTIUM
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DOI：
10.1128/jb.145.1.122-128.1981
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发表时间：
1981-01-01
影响因子：
3.2
通讯作者：
KUSANO, T
中科院分区：
文献类型：
--
作者：
KAMIO, Y;ITOH, Y;KUSANO, T
尸胺作为反刍链球菌（一种严格厌氧细菌）细胞壁肽聚糖的成分存在。添加到生长培养基中的[14C]尸胺被掺入细胞中，并且在肽聚糖部分中发现掺入的总放射性的约70%。当[ 14 C]尸胺标记的肽聚糖制剂被酸水解时，所有14 C计数均以尸胺形式回收。 [14C]尸胺标记的肽聚糖制剂用溶菌酶消化成3个小片段，这些片段具有放射性并且茚三酮反应呈阳性。一个主要斑点是这些片段的复合物，由丙氨酸、谷氨酸、二氨基庚二酸、尸胺、胞壁酸和葡萄糖胺组成。尸胺的 2 个氨基之一与肽聚糖共价连接，另一个是游离的。测定该菌株肽聚糖制剂的化学组成为：L-丙氨酸-D-丙氨酸-D-谷氨酸-内消旋二氨基庚二酸-尸胺-胞壁酸-葡萄糖胺(1.0:1.0:1.0:1.0:1.1:0.9:1.0)。
Cadaverine exists as a component of cell wall peptidoglycan of S. ruminantium, a strictly anaerobic bacterium. [14C]cadaverine added to the growth medium was incorporated into the cells and about 70% of the total radioactivity incorporated was found in the peptidoglycan fraction. When the [14C]cadaverine-labeled peptidoglycan preparation was acid hydrolyzed, all of the 14C counts were recovered as cadaverine. The [14C]cadaverine-labeled peptidoglycan preparation was digested with lysozyme into 3 small fragments which were radioactive and were positive in ninhydrin reaction. One major spot, a compound of the fragments, was composed of alanine, glutamic acid, diaminopimelic acid, cadaverine, muramic acid and glucosamine. One of the 2 amino groups of cadaverine was covalently linked to the peptidoglycan and the other was free. The chemical composition of the peptidoglycan preparation of this strain was determined to be as follows: L-alanine-D-alanine-D-glutamic acid-meso-diaminopimelic acid-cadaverine-muramic acid-glucosamine (1.0:1.0:1.0:1.0:1.1:0.9:1.0).