Detection of human papillomavirus mRNA and cervical cancer cells in peripheral blood of cervical cancer patients with metastasis
Detection of human papillomavirus mRNA and cervical cancer cells in peripheral blood of cervical cancer patients with metastasis
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DOI：
10.1200/jco.1997.15.3.1008
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发表时间：
1997-03-01
影响因子：
45.3
通讯作者：
Tseng, CJ
中科院分区：
文献类型：
--
作者：
Pao, CC;Hor, JJ;Tseng, CJ
目的：探讨IVb期宫颈癌远处脏器转移患者外周血循环中宫颈癌细胞的存在情况。患者及方法：采用巢式聚合酶链反应（nested polymerase chain reaction， PCR）对15例IVb期宫颈癌转移患者的宫颈癌组织进行人乳头瘤病毒（HPV） 16型DNA的检测。通过反转录和PCR分析了15例癌症患者外周血中HPV 16型e6转化基因转录产物的存在。结果：15例宫颈癌患者中有13例（86.7%）的宫颈癌组织中含有HPV 16型DNA。13例宫颈HPV dna阳性患者中有12例（92.3%）外周血标本含有HPV特异性mRNA，可通过逆转录（RT）和PCR检测。2例宫颈HPV阴性癌患者和12例正常对照者的外周血标本均未检测到HPV 16型e6转化基因mRNA。在转移的宫颈癌患者外周血中检测到的HPV特异性mRNA最有可能的来源是子宫颈来源或脱落的宫颈癌细胞，外周血中HPV E6 mRNA的存在可能是循环宫颈癌细胞的敏感指标，如果PCR阳性被证明能够可靠地预测疾病进展，这些发现可能在宫颈癌和许多其他癌症的治疗中具有临床应用价值。(C) 1997年由美国临床肿瘤学会。
Purpose: To determine the presence of cervical cancer cells in circulating peripheral blood of stage IVb cervical cancer patients with metastasis to distant organs,Patients and Methods: Cervical cancer tissue from 15 stage IVb cervical cancer patients with metastasis were analyzed for the presence of human papillomavirus (HPV) type 16 DNA by nested polymerase chain reaction (PCR). The presence of transcriptional products of the HPV type 16 E6-transforming gene in the peripheral blood of the same 15 cancer patients was analyzed by reverse transcription and PCR. Cervical tissues and peripheral-blood specimens from 12 normal healthy individuals served as controls,Results: Thirteen of 15 (86.7%) cervical cancer tissues from same number of patients were found to contain HPV type 16 DNA. Peripheral-blood specimens from 12 of 13 (92.3%) cervical HPV DNA-positive patients were found to contain HPV-specific mRNA detectable by reverse transcription (RT) and PCR. Cervical tissues from all 12 normal controls were HPV-free, None of the peripheral-blood specimens from two cervical HPV-negative cancer patients and 12 normal controls contained detectable amounts of mRNA of HPV type 16 E6-transforming gene,Conclusion: The most likely source of the HPV-specific mRNA detected in the peripheral blood of cervical cancer patients with metastasis is the cervical cancer cells derived from or shed from the cervix, The presence of HPV E6 mRNAs in peripheral blood may be a sensitive indicator of circulating cervical cancer cells, if PCR positivity is proven to be able to predict disease progression reliably, these findings may have clinical applications in the treatment of cervical and many other cancers, (C) 1997 by American Society of Clinical Oncology.