DIVERSITY OF V3 REGION SEQUENCES OF HUMAN IMMUNODEFICIENCY VIRUSES TYPE-1 FROM THE CENTRAL-AFRICAN-REPUBLIC
DIVERSITY OF V3 REGION SEQUENCES OF HUMAN IMMUNODEFICIENCY VIRUSES TYPE-1 FROM THE CENTRAL-AFRICAN-REPUBLIC
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DOI：
10.1089/aid.1993.9.997
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发表时间：
1993-10-01
影响因子：
1.5
通讯作者：
GIRARD, M
中科院分区：
文献类型：
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作者：
MURPHY, E;KORBER, B;GIRARD, M
从与来自中非共和国班吉的29名AIDS患者的SupT 1细胞共培养的淋巴细胞中获得gp 120的中心部分的核苷酸序列，包括第三高变（V3）环。这些序列表现出显着更大的多样性（平均距离，23%）比以前观察到的分离株从限制性地理区域。发现了属于HIV-1的四种主要亚型的分离株;唯一没有代表的亚型是北美/欧洲亚型B。在扎伊尔和乌干达，亚型A和亚型D几乎占所有HIV-1分离株的比例相等，与此不同，中非共和国的主要亚型是亚型A（10个分离株）和亚型E（9个分离株），占分离株的三分之二。亚型E代表了一组以前仅在泰国发现的变体。仅发现一株属于D亚型的分离株，还发现了两株C亚型的分离株，这是一种与南部非洲和印度分离株相关的亚型，但以前在中部非洲未检测到。这些分离株虽然与C亚型明显聚类，但形成了一个独特的子集，彼此之间的差异为8.8%，与印度和南非的C亚型分离株的差异平均为22.5%。在CAR亚型A（平均成对差异，19.3%）和亚型E（10.9%）分离株中也观察到高患者间、亚型内变异。V3序列的多样性在这组依赖于识别V3的免疫方案的影响。这项研究强调了基于干预策略的知识，在目标人群或地理区域的特定序列的必要性。
Nucleotide sequences of the central portion of gp120, including the third hypervariable (V3) loop, were obtained from lymphocytes cocultivated with SupT1 cells from 29 AIDS patients in Bangui, Central African Republic. These sequences displayed significantly greater diversity (average distance, 23%) than has been previously observed in isolates from comparably restricted geographical areas. Isolates belonging to four major subtypes of HIV-1 were found; the only subtype not represented was the North American/European subtype B. Unlike the situation in Zaire and Uganda, where subtypes A and D account equally for virtually all isolates of HIV-1, the predominant subtypes in the Central African Republic, accounting for two-thirds of the isolates, were subtypes A (10 isolates) and E (9 isolates). Subtype E represents a group of variants that have previously been found only in Thailand. Only one isolate belonging to subtype D was found. Also recovered were two isolates of subtype C, a subtype associated with southern African and Indian isolates but not previously detected in central Africa. These isolates, although clearly clustering with subtype C, formed a distinct subset, differing from one another by 8.8% and from the Indian and South African subtype C isolates by an average of 22.5%. High interpatient, intrasubtype variation was also seen among the CAR subtype A (average pairwise difference, 19.3%) and subtype E (10.9%) isolates. The diversity of V3 sequences in this set has implications for immunization protocols that rely on the recognition of V3. This study underscores the necessity of basing intervention strategies on knowledge of the particular sequences present in the target population or geographical area.