Development of a New Bone-Mimetic Surface Treatment Platform: Nanoneedle Hydroxyapatite (nnHA) Coating
Development of a New Bone-Mimetic Surface Treatment Platform: Nanoneedle Hydroxyapatite (nnHA) Coating
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DOI：
10.1002/adhm.202001102
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发表时间：
2020-10-27
影响因子：
10
通讯作者：
Hoey, David A.
中科院分区：
文献类型：
--
作者：
Eichholz, Kian F.;Von Euw, Stanislas;Hoey, David A.
骨骼的层次结构在驱动细胞行为和组织再生方面发挥着关键作用，在设计骨科应用材料时必须考虑这一点。在此，目的是通过使用熔融电写入来制造用板状（pHA）或新型纳米针状（nnHA）晶体修饰的纤维微结构来重现天然骨环境。核磁共振光谱、扫描电子显微镜、透射电子显微镜和 X 射线衍射证明这些涂层复制了天然骨的纳米结构和成分。使用 pHA 支架，人间充质干/基质细胞 (MSC) 矿化显着增加 5 倍，使用 nnHA 支架，显着增加 14 倍。鉴于矿物质的蛋白质稳定特性，这些材料可通过骨形态发生蛋白 2 (BMP2) 进一步功能化。 nnHA 处理促进 BMP2 的受控释放，进一步增强 MSC 矿物质沉积。最后，证明了这种 nnHA 处理方法的多功能性，可用于涂覆不同的架构/材料，包括熔融沉积成型 (FDM) 支架和 Ti6Al4V 钛。因此，本研究概述了一种制造具有精确纤维微结构和仿骨 nnHA 纤维外涂层的支架的方法，该涂层可显着增强 MSC 成骨和治疗性蛋白质递送，并利用这些结果来展示如何将这种表面处理方法应用于更广泛的多种骨科应用领域。
The hierarchical structure of bone plays pivotal roles in driving cell behavior and tissue regeneration and must be considered when designing materials for orthopedic applications. Herein, it is aimed to recapitulate the native bone environment by using melt electrowriting to fabricate fibrous microarchitectures which are modified with plate-shaped (pHA) or novel nanoneedle-shaped (nnHA) crystals. Nuclear magnetic resonance spectroscopy, scanning electron microscopy, transmission electron microscopy, and X-ray diffraction demonstrate that these coatings replicate the nanostructure and composition of native bone. Human mesenchymal stem/stromal cell (MSC) mineralization is significantly increased fivefold with pHA scaffolds and 14-fold with nnHA scaffolds. Given the protein stabilizing properties of mineral, these materials are further functionalized with bone morphogenetic protein 2 (BMP2). nnHA treatment facilitates controlled release of BMP2 which further enhance MSC mineral deposition. Finally, the versatility of this nnHA treatment method, which may be used to coat different architectures/materials including fused deposition modeling (FDM) scaffolds and Ti6Al4V titanium, is demonstrated. This study thus outlines a method for fabricating scaffolds with precise fibrous microarchitectures and bone-mimetic nnHA extrafibrillar coatings which significantly enhance MSC osteogenesis and therapeutic protein delivery, and leverages these results to show how this surface treatment method may be applied to a wider field for multiple orthopedic applications.