HCV RNA in patients with chronic hepatitis C treated with interferon-alpha.
HCV RNA in patients with chronic hepatitis C treated with interferon-alpha.
复制标题
使用干扰素-α 治疗的慢性丙型肝炎患者的 HCV RNA。
DOI：
10.1002/jmv.1890400114
复制
发表时间：
1993
影响因子：
12.7
通讯作者：
Blum,HE
中科院分区：
文献类型：
--
作者：
Liang,TJ;Rustgi,V;Galun,E;Blum,HE
为评价干扰素-α治疗慢性丙型肝炎的疗效，采用半定量聚合酶链式反应方法检测了16例慢性丙型肝炎患者治疗前后血清和肝组织中丙型肝炎病毒核糖核酸水平。用四抗原重组免疫印迹试验(4-RIBA)检测16例患者中有15例抗-HCV抗体阳性。只有两名患者的ALT在干扰素治疗后恢复正常；三名患者出现部分反应。部分应答组中只有一名患者在治疗后显示出明显的丙型肝炎病毒RNA水平下降。在无应答组中，几名患者虽然ALT仍然升高，但对干扰素的反应显示血清或肝脏中的丙型肝炎病毒RNA水平显著下降。我们的数据表明，干扰素治疗慢性丙型肝炎可能不能有效根除丙型肝炎病毒感染，丙氨酸氨基转移酶的降低很少与血清或肝脏中丙型肝炎病毒RNA水平的降低相关。停止治疗经常与丙型肝炎病毒复制和疾病的复发有关。©1993 Wiley-Liss，Inc.
In order to assess the efficacy of interferon‐α on hepatitis C viral RNA in patients with chronic hepatitis C, we analyzed the levels of HCV RNA in sera and liver tissues of 16 patients pre‐ and posttreatment using a semiquantitative polymerase chain reaction method. Fifteen of 16 patients were positive for anti‐HCV antibodies by four‐antigen recombinant immunoblot assay (4‐RIBA). Only two patients demonstrated normalization of ALT in response to interferon; three patients showed a partial response. Only one patient from the partial responder group displayed a significant reduction of HCV RNA level posttreatment. In the nonresponder group, several patients, although their ALTs remained elevated, demonstrated significant decreases in HCV RNA levels in either serum or liver in response to interferon. Our data suggest that interferon treatment of chronic hepatitis C may not be effective in eradicating HCV infection and a reduction in ALT is infrequently associated with a decrease in HCV RNA level in either serum or liver. Cessation of treatment is frequently associated with the recrudescence of HCV replication and disease. © 1993 Wiley‐Liss, Inc.