Degree of endothelium injury promotes fibroelastogenesis in experimental acute lung injury
Degree of endothelium injury promotes fibroelastogenesis in experimental acute lung injury
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DOI：
10.1016/j.resp.2010.08.005
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发表时间：
2010-09-30
影响因子：
2.3
通讯作者：
Rocco, Patricia R. M.
中科院分区：
文献类型：
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作者：
Chao, Monica C. M.;Garcia, Cristiane S. N. B.;Rocco, Patricia R. M.
我们测试了以下假设：在急性肺损伤（ALI）的早期阶段，内皮损伤的程度可以预测肺实质重塑。为此，在小鼠中开发了两种由大肠杆菌诱导的肺外 ALI 模型：脂多糖（ALI-LPS）或盲肠结扎穿刺（ALI-CLP）。在第 1 天，这些模型具有相似程度的肺机械损伤、上皮损伤和腹膜内炎症，但ALI-CLP 中内皮损伤更大 时间过程分析显示，第 7 天时，ALI-CLP 具有更高程度的上皮损伤、基底膜剥脱、内皮损伤、弹性纤维和胶原纤维含量、支气管肺泡灌洗液 (BALF)、腹膜液和血液中的中性粒细胞，白细胞介素 6、KC（IL-8 的小鼠类似物）和转化生长因子 β 的水平BALF 相反，两组肺凋亡细胞数量相似。 总之，除了上皮损伤和腹膜内炎症的维持外，弹力纤维生成的强度还受到内皮损伤的影响。 (C) 2010 Elsevier B V 版权所有
We tested the hypothesis that at the early phase of acute lung injury (ALI) the degree of endothelium injury may predict lung parenchyma remodelling For this purpose, two models of extrapulmonary ALI induced by Escherichia col: lipopolysaccharide (ALI-LPS) or cecal ligation and puncture (ALI-CLP) were developed in mice At day 1, these models had similar degrees of lung mechanical compromise, epithelial damage, and intraperitoneal inflammation, but endothelial lesion was greater in ALI-CLP A time course analysis revealed, at day 7 ALI-CLP had higher degrees of epithelial lesion, denudation of basement membrane, endothelial damage, elastic and collagen fibre content, neutrophils in bronchoalveolar lavage fluid (BALF), peritoneal fluid and blood, levels of interleukin-6, KC (murine analogue of IL-8), and transforming growth factor-beta in BALF Conversely, the number of lung apoptotic cells was similar in both groups In conclusion, the intensity of fibroelastogenesis was affected by endothelium injury in addition to the maintenance of epithelial damage and intraperitoneal inflammation. (C) 2010 Elsevier B V All rights reserved