Insulators and domains of gene expression
Insulators and domains of gene expression
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DOI：
10.1016/j.gde.2015.11.009
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发表时间：
2016-04-01
影响因子：
4
通讯作者：
Bartkuhn, Marek
中科院分区：
文献类型：
--
作者：
Ali, Tamer;Renkawitz, Rainer;Bartkuhn, Marek
基因组组织成活性和非活性染色质结构域对具有结构域边界提出了特定要求，以禁止相邻结构域的相反活性之间的干扰。这些边界通过结合介导长程相互作用的结构蛋白质来提供绝缘子功能。其中，CTCF通过募集粘附素在建立染色质环（CTCF结合位点对之间）中起着突出的作用。CTCF介导的长程相互作用对于间期染色质的许多拓扑特征是不可或缺的，例如拓扑相关结构域的形成、结构域隔离、增强子阻断甚至增强子功能。
The genomic organization into active and inactive chromatin domains imposes specific requirements for having domain boundaries to prohibit interference between the opposing activities of neighbouring domains. These boundaries provide an insulator function by binding architectural proteins that mediate long-range interactions. Among these, CTCF plays a prominent role in establishing chromatin loops (between pairs of CTCF binding sites) through recruiting cohesin. CTCF-mediated long-range interactions are integral for a multitude of topological features of interphase chromatin, such as the formation of topologically associated domains, domain insulation, enhancer blocking and even enhancer function.