Preparation of Cu(II)-Chelated Poly(vinyl alcohol) Nanofibrous Membranes for Catalase Immobilization
Preparation of Cu(II)-Chelated Poly(vinyl alcohol) Nanofibrous Membranes for Catalase Immobilization
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DOI：
10.1002/app.33493
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发表时间：
2011-06-15
影响因子：
3
通讯作者：
Wei, Anjing
中科院分区：
文献类型：
--
作者：
Feng, Quan;Xia, Xin;Wei, Anjing
采用静电纺丝法制备了聚乙烯醇（PVA）纳米纤维。通过Cu（II）溶液与纳米纤维反应制备了金属螯合纳米纤维膜，并将其用作过氧化氢酶的固定化基质。本文研究了固定化过氧化氢酶对铜（II）的吸附常数和性质。采用原子吸收分光光度计（AAS）测定Cu（II）浓度，傅立叶变换红外光谱（FTIR）对固定化酶进行表征，紫外分光光度计（UV）采用Bradford法测定固定化酶的量。采用Langmuir等温吸附模型研究了PVA纳米纤维对Cu（II）的吸附。配位Cu（II）的最大量（q（m））为2.1 mmol g（-1）（干纤维），结合常数（K-1）为0.1166 L mmol（-1）。固定化过氧化氢酶比游离过氧化氢酶具有更好的耐pH和耐温度失活能力，其热稳定性和贮存稳定性也比游离过氧化氢酶高。动力学参数进行了分析固定化和游离过氧化氢酶。固定化过氧化氢酶的Vmax（8425.8 μ mol mg（-1））小于游离过氧化氢酶的Vmax（10153.6 μ mol mg（-1）），而Km值大于游离过氧化氢酶。结果表明，PVA-Cu（II）螯合纳米纤维膜在酶固定化和生物传感器方面具有良好的应用前景。(C)2011 Wiley Periodicals，Inc. J Appl Polym Sci 120：3291-3296，2011
Poly(vinyl alcohol) (PVA) nanofibers were formed by electrospinning. Metal chelated nanofibrous membranes were prepared by reaction between Cu(II) solution and nanofibers, and which were used as the matrix for catalases immobilization. The constants of Cu(II) adsorption and properties of immobilized catalases were studied in this work. The Cu(II) concentration was determined by atomic absorption spectrophotometer (AAS), the immobilized enzymes were confirmed by the Fourier transform infrared spectroscopy (FTIR), and the amounts of immobilized enzymes were determined by the method of Bradford on an ultraviolet spectrophotometer (UV). Adsorption of Cu(II) onto PVA nanofibers was studied by the Langmuir isothermal adsorption model. The maximum amount of coordinated Cu(II) (q(m)) was 2.1 mmol g(-1) (dry fiber), and the binding constant (K-l) was 0.1166 L mmol(-1). The immobilized catalases showed better resistance to pH and temperature inactivation than that of free form, and the thermal and storage stabilities of immobilized catalases were higher than that of free catalases. Kinetic parameters were analyzed for both immobilized and free catalases. The value of V-max (8425.8 mu mol mg(-1)) for the immobilized catalases was smaller than that of the free catalases (10153.6 mu mol mg(-1)), while the K-m for the immobilized catalases were larger. It was also found that the immobilized catalases had a high affinity with substrate, which demonstrated that the potential of PVA-Cu(II) chelated nanofibrous membranes applied to enzyme immobilization and biosensors. (C) 2011 Wiley Periodicals, Inc. J Appl Polym Sci 120: 3291-3296, 2011