Oxidative stress and innate immunity responses in cigarette smoke stimulated nasal epithelial cells
Oxidative stress and innate immunity responses in cigarette smoke stimulated nasal epithelial cells
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DOI：
10.1016/j.tiv.2013.11.004
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发表时间：
2014-03-01
影响因子：
3.2
通讯作者：
Gjomarkaj, Mark
中科院分区：
文献类型：
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作者：
Pace, Elisabetta;Ferraro, Maria;Gjomarkaj, Mark
香烟烟雾提取物（CSE）可能在包括慢性鼻窦炎在内的上呼吸道疾病中发挥重要作用。即使短期接触香烟烟雾也会对线粒体功能和组织氧化还原稳态产生不利影响，并可能发展为与慢性吸烟相关的进一步并发症。香烟烟雾改变支气管上皮细胞中toll样受体4 （TLR4）的表达和激活。碳半胱氨酸是一种抗氧化剂和解粘剂。碳半胱氨酸对CSE诱导的氧化应激以及相关的鼻上皮细胞先天免疫和炎症反应的影响在很大程度上是未知的。本研究旨在评估在CSE刺激的鼻上皮细胞（RPMI 2650）中，碳半胱氨酸（10(-4)M）对细胞存活、细胞内活性氧（ROS）产生、TLR4表达、LPS结合和中性粒细胞趋化（肌动蛋白重组）的影响。我们发现，CSE增加了ROS的产生、TLR4的表达、LPS结合和中性粒细胞趋化性，所有这些事件都被用碳半胱氨酸预孵育CSE刺激的RPMI 2650细胞所抵消。总之，目前的研究提供了令人信服的证据，表明碳半胱氨酸可能被认为是一种有希望的治疗慢性炎症性鼻疾病的策略。(C) 2013 Elsevier Ltd.版权所有。
Cigarette smoke extracts (CSE) may play a significant role in diseases of the upper airway including chronic rhinosinusitis. Even short term exposure of cigarette smoke has adverse effects on mitochondrial functions and redox homeostasis in tissues which may progress to further complications associated with chronic smoking. Cigarette smoke alters toll-like receptor 4 (TLR4) expression and activation in bronchial epithelial cells. Carbocysteine is an anti-oxidant and mucolytic agent. The effects of carbocysteine on CSE induced oxidative stress and on associated innate immune and inflammatory responses in nasal epithelial cells are largely unknown. The present study was aimed to assess in CSE stimulated nasal epithelial cells (RPMI 2650) the effects of carbocysteine (10(-4) M) on: cell survival, intracellular reactive oxygen species (ROS) production, TLR4 expression, LPS binding and neutrophil chemotaxis (actin reorganization). We found that CSE increased ROS production, TLR4 expression, LPS binding and neutrophil chemotaxis and all these events were counteracted by pre-incubating CSE stimulated RPMI 2650 cells with carbocysteine. In conclusion, the present study provides compelling evidence that carbocysteine may be considered a promising therapeutic strategy in chronic inflammatory nasal diseases. (C) 2013 Elsevier Ltd. All rights reserved.