The 'SELection End points in Communities of bacTeria' (SELECT) Method: A Novel Experimental Assay to Facilitate Risk Assessment of Selection for Antimicrobial Resistance in the Environment.
The 'SELection End points in Communities of bacTeria' (SELECT) Method: A Novel Experimental Assay to Facilitate Risk Assessment of Selection for Antimicrobial Resistance in the Environment.
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DOI：
10.1289/ehp6635
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发表时间：
2020-10
影响因子：
10.4
通讯作者：
Gaze WH
中科院分区：
文献类型：
--
作者：
Murray AK;Stanton IC;Wright J;Zhang L;Snape J;Gaze WH
抗菌素耐药性(AMR)是危害人类健康的重要因素之一。越来越多的研究表明，AMR的选择可能发生在抗生素的环境浓度。然而，目前还没有确定抗菌剂选择性浓度的标准化实验方法，这阻碍了对AMR进行适当的环境和人类健康风险评估。我们的目标是设计一种快速、简单、经济有效的新型实验方法来确定抗生素的选择性效应浓度，并产生迄今为止最大的抗生素选择性效应浓度的实验数据集。使用以前发表的方法和数据来验证该分析，该分析基于细菌群落(废水)生长的减少来确定有效浓度。测试抗生素的风险商被生成以量化风险。采用细菌群落选择终点法(SELECT法)快速测定抗生素的选择效应浓度。这与在同一实验系统中测定的定量聚合酶链式反应效应浓度很好地一致。选择方法预测不受检测温度、生长介质或用作接种物的微生物群落变化的影响最小。被测试的抗生素的预测无效浓度范围从环丙沙星到红霉素。缺乏证据表明AMR的环境选择以及相关的人类健康风险，是在减少向水环境中释放抗生素方面缺乏行动的主要原因。我们提出了一种新的方法，可以可靠和快速地填补这一数据空白，以实现监管和随后的缓解(如果需要)，以降低在水环境中选择AMR和人类暴露于AMR的风险。尤其是环丙沙星，以及阿奇霉素、头孢噻肟和甲氧苄啶，在较小程度上都对环境中的AMR的选择构成了重大风险。Https://doi.org/10.1289/EHP6635
Antimicrobial resistance (AMR) is one of the most significant health threats to society. A growing body of research demonstrates selection for AMR likely occurs at environmental concentrations of antibiotics. However, no standardized experimental approaches for determining selective concentrations of antimicrobials currently exist, preventing appropriate environmental and human health risk assessment of AMR. We aimed to design a rapid, simple, and cost-effective novel experimental assay to determine selective effect concentrations of antibiotics and to generate the largest experimental data set of selective effect concentrations of antibiotics to date. Previously published methods and data were used to validate the assay, which determines the effect concentration based on reduction of bacterial community (wastewater) growth. Risk quotients for test antibiotics were generated to quantify risk. The assay (SELection End points in Communities of bacTeria, or the SELECT method) was used to rapidly determine selective effect concentrations of antibiotics. These were in good agreement with quantitative polymerase chain reaction effect concentrations determined within the same experimental system. The SELECT method predicted no effect concentrations were minimally affected by changes in the assay temperature, growth media, or microbial community used as the inoculum. The predicted no effect concentrations for antibiotics tested ranged from for ciprofloxacin to for erythromycin. The lack of evidence demonstrating environmental selection for AMR, and of associated human health risks, is a primary reason for the lack of action in the mitigation of release of antibiotics into the aquatic environment. We present a novel method that can reliably and rapidly fill this data gap to enable regulation and subsequent mitigation (where required) to lower the risk of selection for, and human exposure to, AMR in aquatic environments. In particular, ciprofloxacin and, to a lesser extent, azithromycin, cefotaxime, and trimethoprim all pose a significant risk for selection of AMR in the environment. https://doi.org/10.1289/EHP6635