Effect of modification degree of nanohydroxyapatite on biocompatibility and mechanical property of injectable poly(methyl methacrylate)-based bone cement.
Effect of modification degree of nanohydroxyapatite on biocompatibility and mechanical property of injectable poly(methyl methacrylate)-based bone cement.
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DOI：
10.1002/jbm.b.33428
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发表时间：
2016-04
期刊：
影响因子：
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通讯作者：
Changyun Quan;Yong Tang;Zhenzhen Liu;Minyu Rao;Wei Zhang;Peiqing Liang;Nan Wu;Chao Zhang;Huiyong Shen;Qing Jiang
中科院分区：
文献类型：
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作者：
Changyun Quan;Yong Tang;Zhenzhen Liu;Minyu Rao;Wei Zhang;Peiqing Liang;Nan Wu;Chao Zhang;Huiyong Shen;Qing Jiang
本研究的目的是制备一种生物相容性良好的纳米羟基磷灰石/聚甲基丙烯酸甲酯(HA/PMMA)复合骨水泥，该骨水泥具有良好的力学性能，可用于椎体成形术。将经甲氧基硅烷[P(poly(methylmethacrylate-co-γ-methacryloxypropyl-co-MPS)]共聚物表面修饰的纳米羟基磷灰石(纳米HA)掺入复合骨水泥中，其质量分数可达40wt%。通过热重分析(TGA)测定了P(MMA-co-MPS)在纳米HA表面的含量(18.7%、22.8%和26%)。用扫描电子显微镜观察复合骨水泥生物矿化表面的形态。复合胶凝材料的力学性能测试表明，HA表面改性后，HA与PMMA之间的界面相互作用明显增强。生化检测结果表明，HA/PMMA骨水泥无细胞毒性，无明显溶血作用。细胞黏附和碱性磷酸酶(ALP)活性测定表明，HA/PMMA骨水泥具有良好的生物相容性，可作为一种理想的负重骨修复材料应用于临床。
The objective of this study is to prepare a biocompatible nanohydroxyapatite/poly(methyl methacrylate) (HA/PMMA) composite bone cement, which has good mechanical property and can be used for vertebroplasty. Up to 40 wt % of nanohydroxyapatite (nano-HA) in the power, which was surface modified with poly(methylmethacrylate-co-γ-methacryloxypropyl timethoxysilane) [P(MMA-co-MPS)] copolymer, was incorporated into the composite bone cement. The content of P(MMA-co-MPS) on the surface of nano-HA (18.7%, 22.8%, and 26%) was determined through thermogravimetric analysis (TGA). The morphology of biomineralized surface of composite bone cement was observed under scanning electron microscope (SEM). The mechanical measurements of the composite cements implied that the interfacial interaction between the HA and PMMA matrix may be greatly enhanced after surface modification of HA. Biochemical assays indicated that the HA/PMMA bone cement had no cytotoxicity and induced no hemolysis. The cell adhesion and alkaline phosphatase (ALP) activity assays indicated that the biocompatibility of HA/PMMA bone cement could be promoted, demonstrating that it can be used as an ideal weight-bearing bone repair materials on clinical application.