Hrip1, a novel protein elicitor from necrotrophic fungus, Alternaria tenuissima, elicits cell death, expression of defence-related genes and systemic acquired resistance in tobacco
Hrip1, a novel protein elicitor from necrotrophic fungus, Alternaria tenuissima, elicits cell death, expression of defence-related genes and systemic acquired resistance in tobacco
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DOI：
10.1111/j.1365-3040.2012.02539.x
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发表时间：
2012-12-01
影响因子：
7.3
通讯作者：
Qiu, Dewen
中科院分区：
文献类型：
--
作者：
Kulye, Mahesh;Liu, Hua;Qiu, Dewen
本文报道了一种由坏死性真菌Alternaria tenuissima分泌的新型过敏反应诱导蛋白的鉴定、纯化、表征和基因克隆，该蛋白被命名为过敏反应诱导蛋白1 （hypersensitive response inducing protein 1, Hrip1）。该蛋白引起坏死病变的形成，模仿典型的过敏反应和细胞凋亡相关事件，包括DNA阶梯。采用cDNA末端快速扩增（RACE）方法克隆该蛋白编码基因。序列分析表明，该cDNA全长495 bp，开放阅读框（ORF）编码163个氨基酸的多肽，理论pI为5.50，分子量为17 562.5 Da。Hrip1侵染烟叶后可诱导钙内流、介质碱化、水杨酸诱导的蛋白激酶活化及几种防御相关基因。细胞损伤局限于浸润区，仅在几个小时后发生，此时防御相关基因的表达被激活。几天后，全身获得性耐药也被诱导。感知到Hrip1的烟草植物细胞产生了一系列作用于局部、短距离和远距离的信号，并以类似于对烟草花叶病毒过敏的方式引起特定防御反应的协调表达。因此，Hrip1是进一步研究坏死真菌诱导抗病信号及其转导途径的有力工具。
Here, we report the identification, purification, characterization and gene cloning of a novel hypersensitive response inducing protein secreted by necrotrophic fungus, Alternaria tenuissima, designated as hypersensitive response inducing protein 1 (Hrip1). The protein caused the formation of necrotic lesions that mimic a typical hypersensitive response and apoptosis-related events including DNA laddering. The protein-encoding gene was cloned by rapid amplification of cDNA ends (RACE) method. The sequence analysis revealed that the cDNA is 495 bp in length and the open reading frame (ORF) encodes for a polypeptide of 163 amino acids with theoretical pI of 5.50 and molecular weight of 17 562.5 Da. Hrip1 induced calcium influx, medium alkalinization, activation of salicylic acid-induced protein kinase and several defence-related genes after infiltration in tobacco leaves. Cellular damage, restricted to the infiltrated zone, occurred only several hours later, at a time when expression of defence-related genes was activated. After several days, systemic acquired resistance was also induced. The tobacco plant cells that perceived the Hrip1 generated a cascade of signals acting at local, short, and long distances, and caused the coordinated expression of specific defence responses in a way similar to hypersensitivity to tobacco mosaic virus. Thus, Hrip1 represents a powerful tool to investigate further the signals and their transduction pathways involved in induced disease resistance in necrotrophic fungi.