First certified reference material for cystatin C in human serum ERM-DA471/IFCC
First certified reference material for cystatin C in human serum ERM-DA471/IFCC
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DOI：
10.1515/cclm.2010.318
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发表时间：
2010-11-01
影响因子：
6.8
通讯作者：
Zegers, Ingrid
中科院分区：
文献类型：
--
作者：
Grubb, Anders;Blirup-Jensen, Soren;Zegers, Ingrid
IFCC胱抑素C标准化工作组（WG-SCC）与标准物质和测量研究所（IRMM）合作，宣布提供新的认证标准物质ERM-DA 471/IFCC。使用纯蛋白质一级参比制剂（PRP）作为校准品对材料进行表征。从重组半胱氨酸蛋白酶抑制剂C制备PRP，并使用干质量测定法测量其浓度。通过颗粒增强免疫比浊法、颗粒增强免疫比浊法和酶放大单向免疫扩散进行ERM-DA 471/IFCC的表征。如果按照规定程序复溶，则ERM-DA 471/IFCC中经认证的胱抑素C质量浓度为5.48 mg/L，扩展不确定度（k=2）为0.15 mg/L。临床化学实验室医学2010;48：1619-21。
The IFCC Working Group for the Standardisation of Cystatin C (WG-SCC), in collaboration with the Institute for Reference Materials and Measurements (IRMM), announces the availability of the new certified reference material ERM-DA471/IFCC. The material was characterised using a pure protein primary reference preparation (PRP) as calibrant. The PRP was prepared from recombinant cystatin C, and its concentration measured using dry mass determination. The characterisation of ERM-DA471/IFCC was performed by particle enhanced immuno-nephelometry, particle enhanced immuno-turbidimetry, and enzyme amplified single radial immuno-diffusion. The certified cystatin C mass concentration in ERM-DA471/IFCC, if reconstituted according to the specified procedure, is 5.48 mg/L, the expanded uncertainty (k=2) being 0.15 mg/L. Clin Chem Lab Med 2010;48:1619-21.